LOCUS       sequence-80021-e        2187 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of a Nano-lantern-based luminescent Ca2+ 
            indicator with Kd=300nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(Ca2+)_300/pcDNA3
FEATURES             Location/Qualifiers
     source          1..2187
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(13..15,16..18,19..531)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(58..79)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(319..338)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     666..687
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1846..1923
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 ggatccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
       61 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      121 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
      181 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
      241 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      301 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      361 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      421 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
      481 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
      541 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      601 cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc
      661 gatcacatgg tcctgctgga gttcgtgacc gccgccgggg gtaccaaggt gtacgacccc
      721 gagcagagga agaggatgat caccggcccc cagtggtggg ccaggtgcaa gcagatgaac
      781 gtgctggaca gcttcatcaa ctactacgac agcgagaagc acgccgagaa cgccgtgatc
      841 ttcctgcacg gcaacgccac tagcagctac ctgtggaggc acgtggtgcc ccacatcgag
      901 cccgtggcca ggtgcatcat ccccgatctg atcggcatgg gcaagagcgg caagagcggc
      961 aacggcagct acaggctgct ggaccactac aagtacctga ccgcctggtt cgagctcctg
     1021 aacctgccca agaagatcat cttcgtgggc cacgactggg gcgccgccct ggccttccac
     1081 tacgcctacg agcaccagga caggatcaag gccatcgtgc acatggagag cgtggtggac
     1141 gtgatcgaga gctgggacga gtggccagac atcgaggagg acatcgccct gatcaagagc
     1201 gaggagggcg agaagatggt gctggagaac aacttcttcg tggagaccgt gctgcccagc
     1261 aagatcatga gaaagctgga gcccgaggag ttcgccgcct acctggagcc cttcaaggag
     1321 aagggcgagg tgagaagacc caccctgagc tggcccagag agatccccct ggtgaagggc
     1381 ccatggatgc atgaccaact gacagaagag cagattgcag agttcaaaga agccttctca
     1441 ttattcgaca aggatgggga cggcaccatc accacaaagg aacttggcac cgttatgagg
     1501 tcgcttggac aaaacccaac ggaagcagaa ttgcaggata tgatcaatga agtcgatgct
     1561 gatggcaatg gaacgattta ctttcctgaa tttcttacta tgatggctag aaaaatgaag
     1621 gacacagaca gcgaagagga aatccgagaa gcattccgtg tttttgacaa ggatgggaac
     1681 ggctacatca gcgctgctca gttacgtcac gtcatgacaa acctcgggga gaagttaaca
     1741 gatgaagaag ttgatgaaat gataagggaa gcagatatcg atggtgatgg ccaagtaaac
     1801 tatgaagagt ttgtacaaat gatgacagca aaggggggcg ggtccaagag gcgctggaag
     1861 aaaaacttca ttgccgtcag cgctgccaac cggttcaaga agatctccag ctccggggca
     1921 ctggagctcg gcaagcccga cgtggtgcag atcgtgagaa actacaacgc ctacctgaga
     1981 gccagcgacg acctgcccaa gctgttcatc gagggcgacc ccggcttctt cagcaacgcc
     2041 atcgtggagg gcgccaagaa gttccccaac accgagttcg tgaaggtgaa gggcctgcac
     2101 ttcctccagg aggacgcccc cgacgagatg ggcaagtaca tcaagagctt cgtggagaga
     2161 gtgctgaaga acgagcagta agaattc
//
