LOCUS       sequence-80029-e        2265 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for Nano-lantern-based luminescent ATP 
            indicator, targeted to chloroplasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: CT-Nano-lantern(ATP1)/pENTR1A
FEATURES             Location/Qualifiers
     source          1..2265
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(217..219,220..222,223..735)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(262..283)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(523..542)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     870..891
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcgacatgg attcacagct agtcttgtct ctgaagctga atccaagctt cactcctctt
       61 tctcctctct tccctttcac tccatgttct tctttttcgc cgtcgctccg gttttcttct
      121 tgctactccc gccgcctcta ttctccggtt accgtctacg ccgcgaagaa actctcccac
      181 aaaatcagtt ctgagtttga tcgcgacaag gatccgatgg tgagcaaggg cgaggagctg
      241 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      301 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc
      361 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc
      421 ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      481 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      541 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      601 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
      661 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
      721 cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc
      781 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaagctg
      841 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      901 gggggtacca tgaccagcaa ggtgtacgac cccgagcaga ggaagaggat gatcaccggc
      961 ccccagtggt gggccaggtg caagcagatg aacgtgctgg acagcttcat caactactac
     1021 gacagcgaga agcacgccga gaacgccgtg atcttcctgc acggcaacgc cactagcagc
     1081 tacctgtgga ggcacgtggt gccccacatc gagcccgtgg ccaggtgcat catccccgat
     1141 ctgatcggca tgggcaagag cggcaagagc ggcaacggca gctacaggct gctggaccac
     1201 tacaagtacc tgaccgcctg gttcgagctt ctgaacctgc ccaagaagat catcttcgtg
     1261 ggccacgact ggggcgccgc cctggccttc cactacgcct acgagcacca ggacaggatc
     1321 aaggccatcg tgcacatgga gagcgtggtg gacgtgatcg agagctggga cgagtggcca
     1381 gacatcgagg aggacatcgc cctgatcaag agcgaggagg gcgagaagat ggtgctggag
     1441 aacaacttct tcgtggagac cgtgctgccc agcaagatca tgagaaagct ggagcccgag
     1501 gagttcgccg cctacctgga gcccttcaag gagaagggcg aggtgagaag acccaccctg
     1561 agctggccca gagagatccc cctggtgaag ggcccatgga tgaaaactgt gaaagtgaat
     1621 ataacaaccc ctgatgggcc agtctacgac gctgatatcg agatggtgtc cgtgcgggcc
     1681 gagagtggtg atctcggcat cctccccggt cacattccca caaaggcccc actgaagatc
     1741 ggagctgtgc ggctgaagaa ggacggccaa accgagatgg tcgcagtctc aggcggcact
     1801 gttgaagtgc ggcctgacca cgttaccatt aatgctcaag ccgctgaaac agccgaagga
     1861 atcgacaaag agagagcaga agccgcaaga cagagggccc aggagcggct gaactctcaa
     1921 tccgatgaca ccgatattcg ccgggccgag ctggcactgc agagggccct gaacagactg
     1981 gacgtggctg ggaaggcaaa cgagctcggc aagcccgacg tggtgcagat cgtgagaaac
     2041 tacaacgcct acctgagagc cagcgacgac ctgcccaagc tgttcatcga gggtgacccc
     2101 ggcttcttca gcaacgccat cgtggagggc gccaagaagt tccccaacac cgagttcgtg
     2161 aaggtgaagg gcctgcactt cctccaggag gacgcccccg acgagatggg caagtacatc
     2221 aagagcttcg tggagagagt gctgaagaac gagcagtaag aattc
//
