LOCUS       sequence-80031-e        2056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for Nano-lantern-based luminescent ATP 
            indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(ATP1)/pENTR1A
FEATURES             Location/Qualifiers
     source          1..2056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(8..10,11..13,14..526)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(53..74)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(314..333)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     661..682
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggatccgatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
       61 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      121 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
      181 gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca
      241 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      301 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      361 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      421 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca
      481 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca
      541 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      601 caaccactac ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca
      661 catggtcctg ctggagttcg tgaccgccgc cgggggtacc atgaccagca aggtgtacga
      721 ccccgagcag aggaagagga tgatcaccgg cccccagtgg tgggccaggt gcaagcagat
      781 gaacgtgctg gacagcttca tcaactacta cgacagcgag aagcacgccg agaacgccgt
      841 gatcttcctg cacggcaacg ccactagcag ctacctgtgg aggcacgtgg tgccccacat
      901 cgagcccgtg gccaggtgca tcatccccga tctgatcggc atgggcaaga gcggcaagag
      961 cggcaacggc agctacaggc tgctggacca ctacaagtac ctgaccgcct ggttcgagct
     1021 tctgaacctg cccaagaaga tcatcttcgt gggccacgac tggggcgccg ccctggcctt
     1081 ccactacgcc tacgagcacc aggacaggat caaggccatc gtgcacatgg agagcgtggt
     1141 ggacgtgatc gagagctggg acgagtggcc agacatcgag gaggacatcg ccctgatcaa
     1201 gagcgaggag ggcgagaaga tggtgctgga gaacaacttc ttcgtggaga ccgtgctgcc
     1261 cagcaagatc atgagaaagc tggagcccga ggagttcgcc gcctacctgg agcccttcaa
     1321 ggagaagggc gaggtgagaa gacccaccct gagctggccc agagagatcc ccctggtgaa
     1381 gggcccatgg atgaaaactg tgaaagtgaa tataacaacc cctgatgggc cagtctacga
     1441 cgctgatatc gagatggtgt ccgtgcgggc cgagagtggt gatctcggca tcctccccgg
     1501 tcacattccc acaaaggccc cactgaagat cggagctgtg cggctgaaga aggacggcca
     1561 aaccgagatg gtcgcagtct caggcggcac tgttgaagtg cggcctgacc acgttaccat
     1621 taatgctcaa gccgctgaaa cagccgaagg aatcgacaaa gagagagcag aagccgcaag
     1681 acagagggcc caggagcggc tgaactctca atccgatgac accgatattc gccgggccga
     1741 gctggcactg cagagggccc tgaacagact ggacgtggct gggaaggcaa acgagctcgg
     1801 caagcccgac gtggtgcaga tcgtgagaaa ctacaacgcc tacctgagag ccagcgacga
     1861 cctgcccaag ctgttcatcg agggtgaccc cggcttcttc agcaacgcca tcgtggaggg
     1921 cgccaagaag ttccccaaca ccgagttcgt gaaggtgaag ggcctgcact tcctccagga
     1981 ggacgccccc gacgagatgg gcaagtacat caagagcttc gtggagagag tgctgaagaa
     2041 cgagcagtaa gaattc
//
