LOCUS       sequence-80032-e        1894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of a 'caged' Ca2+ indicator for fluorescent 
            imaging at specific locations and time points.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Matsuda T, Horikawa K, Saito K, Nagai T
  TITLE     Highlighted Ca(2)(+) imaging with a genetically encoded 'caged' 
            indicator.
  JOURNAL   Sci Rep. 2013;3:1398. doi: 10.1038/srep01398.
  PUBMED    23474844
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013"; volume: "3:1398. doi"; pages: " 10.1038/srep01398"
COMMENT     Sequence Label: PA-TNXL/pcDNA3
FEATURES             Location/Qualifiers
     source          1..1894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(8..10,11..13,14..526)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIE"
     CDS             1151..1885
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNWNSHN
                     VYITADKQKNGIKANFKIRHNIE"
ORIGIN
        1 ggatccgatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
       61 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      121 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      181 gcccaccctc gtgaccacct tcagctacgg cgtgcagtgc ttcagccgct accccgacca
      241 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      301 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      361 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      421 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      481 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      541 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      601 caaccactac ctgagccacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      661 catggtcctg ctggagttcg tgaccgccgc ccgcatgctc agcgaggaga tgattgctga
      721 gttcaaagct gcctttgaca tgtttgatgc ggacggtggt ggggacatca gcaccaagga
      781 gttgggcacg gtgatgagga tgctgggcca gaaccccacc aaagaggagc tggatgccat
      841 catcgaggag gtggacgagg atggcagcgg caccatcgac ttcgaggagt tcctggtgat
      901 gatggtgcgc cagatgaaag aggacgccaa gggcaagtct gaggaggagc tggccaactg
      961 cttccgcatc ttcgacaagg acgctaacgg gttcatcgac atcgaggagc tgggtgagat
     1021 tctcagggcc actggggagc acgtcatcga ggaggacata gaagacctca tgaaggattc
     1081 agacaaggac aataacggcc gcattgactt cgatgagttc ctgaagatga tggagggtgt
     1141 gcaggagctc atggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
     1201 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag
     1261 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1321 gatcactctc ggcatggacg agctgtacaa gggtggcagc ggtggcatgg tgagcaaggg
     1381 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1441 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1501 gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1561 gggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
     1621 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1681 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1741 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1801 ctggaacagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
     1861 cttcaagatc cgccacaaca tcgagtaaga attc
//
