LOCUS       sequence-80033-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses the rodent SINE, B2, using the pol III enhancer sequence 
            of the 7SL gene and tagged with the self splicing intron neo 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Kroutter EN, Belancio VP, Wagstaff BJ, Roy-Engel AM
  TITLE     The RNA polymerase dictates ORF1 requirement and timing of LINE and 
            SINE retrotransposition.
  JOURNAL   PLoS Genet. 2009 Apr;5(4):e1000458. doi: 
            10.1371/journal.pgen.1000458. Epub 2009 Apr 24.
  PUBMED    19390602
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2009-04"; volume: "5(4):e1000458. doi"; pages: " 
            10.1371/journal.pgen.1000458. Epub 2009 Apr 24"
COMMENT     Sequence Label: pB2-neo-TET
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(366..382)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(366..382)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(419..439)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(419..437)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(458..474)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(458..474)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(471..493)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    482..498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(506..512,513..530,531..536))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    551..572
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(689..706)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
       61 ttcttgacga gttcttctga ggggatttga gacgtcttat atgtaagtac actgtagctg
      121 tcttcagaca ctccagaaga gggagtcaga tcttgttacg gatggttgtg agccaccatg
      181 tggttgctgg aatttgaact ctggaccttc ggaagagcag tcgggtgctc taacccactg
      241 agccatctca ccagcccggt cggagacgct gcgaaaagct actagaagct gcagggcagt
      301 tacgtcattg gggagcggag gggccacgcc gggcgacgtg agcttggaca cacccacact
      361 gggcggatcc actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc
      421 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      481 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      541 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      601 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      661 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      721 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      781 gggataacgc aggaaagaac atgtgagcaa aa
//
