LOCUS       sequence-80042-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses U6 RNA, using its own pol III enhancer sequence and tagged
            with the self splicing intron neo cassette .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Kroutter EN, Belancio VP, Wagstaff BJ, Roy-Engel AM
  TITLE     The RNA polymerase dictates ORF1 requirement and timing of LINE and 
            SINE retrotransposition.
  JOURNAL   PLoS Genet. 2009 Apr;5(4):e1000458. doi: 
            10.1371/journal.pgen.1000458. Epub 2009 Apr 24.
  PUBMED    19390602
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2009-04"; volume: "5(4):e1000458. doi"; pages: " 
            10.1371/journal.pgen.1000458. Epub 2009 Apr 24"
COMMENT     Sequence Label: pU6-neo-TET
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(208..456)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(266..285)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(436..456)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(617..633)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(617..633)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(670..690)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(670..688)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(709..725)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(709..725)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(722..744)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    733..749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(757..763,764..781,782..787))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    802..823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
       61 tatcgccttc ttgacgagtt cttctgaggg gatttgagac gtcaatatgg aacgcttcac
      121 gaatttgcgt gtcatccttg cgcaggggcc atgctaatct tctctgtatc gttccaattt
      181 tagtatatgt gctgccgaag cgagcacggt gtttcgtcct ttccacaaga tatataaagc
      241 caagaaatcg aaatactttc aagttacggt aagcatatga tagtccattt taaaacataa
      301 ttttaaaact gcaaactacc caagaaatta ttactttcta cgtcacgtat tttgtactaa
      361 tatctttgtg tttacagtca aattaattct aattatctct ctaacagcct tgtatcgtat
      421 atgcaaatat gaaggaatca tgggaaatag gccctcttcc tgcccgacct tggcgcgcgc
      481 tcggcgcgcg gtcacgctcc gtcacgtggt gcgttttgcc tgcgcgtctt tccactgggg
      541 ccggggcggc gcggagggtt cagacgcggg ctcttactca aacgctgatc cccgaaacta
      601 ccgagcagtg tctgcggatc cactagttct agagcggccg ccaccgcggt ggagctccag
      661 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
      721 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
      781 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
      841 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taat
//
