LOCUS       sequence-80046-e         506 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 
            mutated to Ala.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel 
            MN, Novitch BG, Rohatgi R
  TITLE     Gli Protein Activity Is Controlled by Multisite Phosphorylation in 
            Vertebrate Hedgehog Signaling.
  JOURNAL   Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003.
  PUBMED    24373970
REFERENCE   2  (bases 1 to 506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003."
COMMENT     Sequence Label: pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A
FEATURES             Location/Qualifiers
     source          1..506
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..48)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    101..125
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(143..166)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctt cgaaccgcgg
       61 gccctctaga ctcgagcggc cgccactgtg ctggatatca accactttgt acaagaaagc
      121 tgggtctaga tatctcgact cacttgtcat cgtcgtcctt gtaatctccg ccagatcctg
      181 gtgttccgcc acgcgtctgc ataactgcaa ggaacttgct ttcttctgca agggaggtca
      241 gcaaagaact catatccccg atagccatgt tggtggtgcc catggatagg gatgggaatg
      301 ggagggatgc ccgcggagtg gtgagacggg aggagctgtg ggaaaggttc tgaataatac
      361 ttgggctcaa ggcccctgac attaggctgg tatggtcccc atcatctatg atggcatcaa
      421 aatcaatctg cacaccttct aggtcatgac tctcaaagct gtcaactgtg cttgttacct
      481 ggttagcacc tggggagagt aactca
//
