LOCUS       sequence-80056-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with attL1 and attR5 sites. Between the att 
            sites are two FokI coding sequences transcriptionally linked by a 
            T2A sequence. There is a nos-T after the last FokI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pNJB91
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..588)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     CDS             complement(629..649)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(656..721)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(728..781)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 aaagtttatc tcgccgttat taaatttccg tctcacttcc tctaaggtta atgtgccggc
       61 tttaatcatt tctccaccaa ttaaaagctc ttctacacta agaacagctc cattacaatt
      121 agtgatatga tttaatcgtg taagctgagc tttgtagttt cctttaaagt gaccactcac
      181 aaataaaaac ttaaattccg ttacagaaga tggatagact ttccaccatt cattagggtt
      241 gagatgtttg tttcgtgttt gattttcttc gacatatcgc tccatttcat ctgcttggcc
      301 aattggcaga ttataacctc cgctataagc tttagtatcc acgatcacac cgtaatcaat
      361 aggagatccg acagtataaa ttgctccgtc cggtttcctt gatccaccca aatgtttacc
      421 tctatatcca taaactttca taaaaaattc cattaccttc atttcaagaa ttctatcctg
      481 agtggaattt ctggcaattt caattaattc aatatattca tgaggcacat atttcaattt
      541 atgacgaagt tcagatttct tctcctccag ttcacttttg actagttgag atctaagctt
      601 actagctagc cggcaccccg tgaatgccca ccttcctctt cttcttgggg gccatcttgt
      661 catcgtcatc cttgtaatcg atgtcatgat ctttataatc accgtcatgg tctttgtagt
      721 ccatcctagg gccgggattc tcctccacgt caccgcatgt tagaagactt cctctgccct
      781 ctccgccgcc ggacctgaaa ttgatttcac cattgttgaa cttgcggcgc acctcttcca
      841 gggtcagggt accagccttg atcatctcgc cgccgatcag cagttcctcc acggacagca
      901 cggcgccgtt gcagttggtc ttgtggttca ggcgggtcag ttgggcctta taattgccct
      961 tgaagtggcc ggacacgaac aggaatttga actcg
//
