LOCUS       sequence-80057-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic vector expressing mRuby2 and GCaMP6s from a single open 
            reading frame..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Rose T, Jaepel J, Hubener M, Bonhoeffer T
  TITLE     Cell-specific restoration of stimulus preference after monocular 
            deprivation in the visual cortex.
  JOURNAL   Science. 2016 Jun 10;352(6291):1319-22. doi: 
            10.1126/science.aad3358.
  PUBMED    27284193
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-06-10- 10"; volume: "352"; 
            issue: "6291"; pages: "1319-22"
COMMENT     Sequence Label: pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      436..445
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cctgacgacc gaccccgacc cactggacaa gcacccaacc cccattcccc aaattgcgca
       61 tcccctatca gagaggggga ggggaaacag gatgcggcga ggcgcgtgcg cactgccagc
      121 ttcagcaccg cggacagtgc cttcgccccc gcctggcggc gcgcgccacc gccgcctcag
      181 cactgaaggc gcgctgacgt cactcgccgg tcccccgcaa actccccttc ccggccacct
      241 tggtcgcgtc cgcgccgccg ccggcccagc cggaccgcac cacgcgaggc gcgagatagg
      301 ggggcacggg cgcgaccatc tgcgctgcgg cgccggcgac tcagcgctgc ctcagtctgc
      361 ggtgggcagc ggaggagtcg tgtcgtgcct gagagcgcag tcgaattcaa gctgctagct
      421 agtaccggtc ctagggccac catggtgtct aagggcgaag agctgatcaa ggaaaatatg
      481 cgtatgaagg tggtcatgga aggttcggtc aacggccacc aattcaaatg cacaggtgaa
      541 ggagaaggca atccgtacat gggaactcaa accatgagga tcaaagtcat cgagggagga
      601 cccctgccat ttgcctttga cattcttgcc acgtcgttca tgtatggcag ccgtactttt
      661 atcaagtacc cgaaaggcat tcctgatttc tttaaacagt cctttcctga gggttttact
      721 tgggaaagag ttacgagata cgaagatggt ggagtcgtca ccgtcatgca ggacaccagc
      781 cttgaggatg gctgtctcgt ttaccacgtc caagtcagag gggtaaactt tccctccaat
      841 ggtcccgtga tg
//
