LOCUS       sequence-80058-e        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with attL1 and attR5 sites. Between the att 
            sites are two FokI coding sequences transcriptionally linked by a 
            T2A sequence. There is a nos-T after the last FokI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pNJB91
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..39
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     31..48
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     32..48
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    64..163
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             185..250
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             257..277
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             918..971
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             978..1043
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1050..1070
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cttgcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
       61 ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag
      121 caatgctttt ttataatgcc aactttgtac aaaaaagcag gctccgaatt cgcccttcac
      181 catggattat aaggatcacg atggcgacta caaggaccac gatattgact acaaagacga
      241 tgacgataaa atggctccta agaaaaagcg caaagtcggt atccatggcg ttccctctag
      301 ataacgcagg atcccagctg gtgaagtccg agctggaaga aaaaaagagc gagctgcgcc
      361 acaagctcaa gtacgtgccc cacgagtaca tcgagctgat cgagatcgcc cgcaacagca
      421 cccaagaccg catcctggag atgaaagtga tggagttctt catgaaggtg tacggctacc
      481 gcggcaagca cctgggcggc tcccgcaagc ccgatggcgc catctacacc gtgggctccc
      541 ccatcgacta tggcgtcatt gtcgacacca aggcctactc cggcggctac aacttaccca
      601 tcggtcaggc cgacgagatg caacgctacg tgaaggagaa ccagacccgc aataagcaca
      661 ttaatcccaa cgagtggtgg aaggtgtacc cctcctccgt gaccgagttc aaattcctgt
      721 tcgtgtccgg ccacttcaag ggcaattata aggcccaact gacccgcctg aaccacaaga
      781 ccaactgcaa cggcgccgtg ctgtccgtgg aggaactgct gatcggcggc gagatgatca
      841 aggctggtac cctgaccctg gaagaggtgc gccgcaagtt caacaatggt gaaatcaatt
      901 tcaggtccgg cggcggagag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga
      961 atcccggccc taggatggac tacaaagacc atgacggtga ttataaagat catgacatcg
     1021 attacaagga tgacgatgac aagatggccc ccaagaagaa gaggaaggtg ggcattcacg
     1081 gggtgccggc tagctagtaa gcttagat
//
