LOCUS       sequence-80060-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic vector expressing mRuby2 and GCaMP6s from a single open 
            reading frame..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Rose T, Jaepel J, Hubener M, Bonhoeffer T
  TITLE     Cell-specific restoration of stimulus preference after monocular 
            deprivation in the visual cortex.
  JOURNAL   Science. 2016 Jun 10;352(6291):1319-22. doi: 
            10.1126/science.aad3358.
  PUBMED    27284193
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-06-10- 10"; volume: "352"; 
            issue: "6291"; pages: "1319-22"
COMMENT     Sequence Label: pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     605..624
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tttgtaatcc agaggttgat tatcgataag ctctagtgcg gccgcttact tcgctgtcat
       61 catttgtaca aactcttcgt agtttacctg accatcccca tcgatgtctg cttccctgat
      121 catttcatca acctcttcat ctgttaactt ctctccaagg tttgtcatca cgtggcgaag
      181 ctctgctgca ctgatgtagc cattgccatc cttatcaaac acaccgaacg cttctctaat
      241 ttcttcttcc gtgtccctgt atttcatttt tcttgccatc attgtcagga actcagggaa
      301 gtcgattgtg ccgtcaccgt cggcatctac ttcattgatc atgtcctgca gctctgcttc
      361 tgtggggttc tgccccagag accgcatcac cgtccccagc tccttggttg ttattgtccc
      421 atccccgtcc ttgtcaaata gggagaaagc ctctttaaat tctgcgatct gctcttcagt
      481 cagttggtcc ggcaggttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
      541 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
      601 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga tgtagccttc
      661 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca
      721 ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
      781 ggtgcagatg aacttcaggg tcagct
//
