LOCUS       sequence-80062-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic vector expressing mRuby2 and GCaMP6s from a single open 
            reading frame..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Rose T, Jaepel J, Hubener M, Bonhoeffer T
  TITLE     Cell-specific restoration of stimulus preference after monocular 
            deprivation in the visual cortex.
  JOURNAL   Science. 2016 Jun 10;352(6291):1319-22. doi: 
            10.1126/science.aad3358.
  PUBMED    27284193
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-06-10- 10"; volume: "352"; 
            issue: "6291"; pages: "1319-22"
COMMENT     Sequence Label: pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(93..114)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(354..373)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcactctcgg catggacgag ctgtacaagg gcggtaccgg agggagcatg gtgagcaagg
       61 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      121 gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc cacctacggc aagctgaccc
      181 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      241 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      301 tcaagtccgc catgcccgaa ggctacatcc aggagcgcac catcttcttc aaggacgacg
      361 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      421 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      481 acctgccgga ccaactgact gaagagcaga tcgcagaatt taaagaggct ttctccctat
      541 ttgacaagga cggggatggg acaataacaa ccaaggagct ggggacggtg atgcggtctc
      601 tggggcagaa ccccacagaa gcagagctgc aggacatgat caatgaagta gatgccgacg
      661 gtgacggcac aatcgacttc cctgagttcc tgacaatgat ggcaagaaaa atgaaataca
      721 gggacacgga agaagaaatt agagaagcgt tcggtgtgtt tgataaggat ggcaatggct
      781 acatcagtgc agcagagctt cgccacgtga tgacaaa
//
