LOCUS       sequence-80065-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic vector expressing mRuby2 and GCaMP6f from a single open 
            reading frame. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Rose T, Jaepel J, Hubener M, Bonhoeffer T
  TITLE     Cell-specific restoration of stimulus preference after monocular 
            deprivation in the visual cortex.
  JOURNAL   Science. 2016 Jun 10;352(6291):1319-22. doi: 
            10.1126/science.aad3358.
  PUBMED    27284193
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-06-10- 10"; volume: "352"; 
            issue: "6291"; pages: "1319-22"
COMMENT     Sequence Label: pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     606..625
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gctctagtgc ggccgcttac ttcgctgtca
       61 tcatttgtac aaactcttcg tagtttacct gaccatcccc atcgatgtct gcttccctga
      121 tcatttcatc aacctcttca tctgttaact tctctccaag gtttgtcatc acgtggcgaa
      181 gctctgctgc actgatgtag ccattgccat ccttatcaaa cacaccgaac gcttctctaa
      241 tttcttcttc cgtgtccctg tatttcattt ttcttgccat cattgtcagg aactcaggga
      301 agtcgattgt gccgtcaccg tcggcatcta cttcattgat catgtcctgc agctctgctt
      361 ctgtggggtt ctgccccaga gaccgcatca ccgtccccag ctccttggtt gttattgtcc
      421 catccccgtc cttgtcaaat agggagaatt cctctttaaa ttctgcgatc tgctcttcag
      481 tcagttggtc cggcaggttg tactccagct tgtgccccag gatgttgccg tcctccttga
      541 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
      601 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg atgtagcctt
      661 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
      721 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
      781 tggtgcagat gaactt
//
