LOCUS       sequence-80069-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pMDC32 with Rep/RepA coding sequence from the mild bean yellow dwarf
            virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pREP
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..19
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8..30
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     27..43
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..43
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(60..312)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    376..400
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaattcccg
       61 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
      121 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
      181 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
      241 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
      301 gtttgaacga tcggggaaat tcgagctcca ccgcggtggc ggccgctcta gaactagtta
      361 attaagaatt atcgaaccac tttgtacaag aaagctgggt cgaattcagg cacgttcagt
      421 gactcgacga ttcccgagca aaaaaagtct ccccgtcaca catgtagtgg gtgacgcaat
      481 tatctttaaa gtaatccttc tgttgacttg tcattgataa catccagtct tcgtcaggat
      541 tgcaaagaat tatagaaggg atcccacctt ttattttctt cttttttcca tatttagggt
      601 tgacagtgaa atcagactgg caacctatta attgcttcca caatgggacg aacttgaagg
      661 ggatgtcgtc gatgatatta taggtggcgt gttcatcgta gttggtgaaa tcgatggtac
      721 cgttccaata gttgtgtcgt ccgagacttc tagcccaggt ggtctttccg gtacgagttg
      781 gtccgcagat gtagaggctg gggtgtcgga ttccattcct tccattgtcc tggttaaatc
      841 ggccatccat tcaaggtcag attgagcttg ttggtatgag acaggatgta tgtaagtata
      901 agcgtctatg cttacatggt atagatgggt ttccctccag gagtgtagat cttcgtggca
      961 gcgaagatct gattctgtga agggcgacac atacggt
//
