LOCUS       sequence-80070-e         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplies the selection marker (eCFP) and IRES with flanked by AttR2 
            and AttL3 sites for Gateway cloning recombination reaction. (This 
            corresponds to a module 3 plasmid)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pBEG R2-eCFP-L3   #BGV202
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    57..519
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(217..236)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     446..463
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ccttgctcac cattctagat gtctcctcag cggatctggc catggttgtg gccatattat
       61 catcgtgttt ttcaaaggaa aaccacgtcc ccgtggttcg gggggcctag acgttttttt
      121 aacctcgact aaacacatgt aaagcatgtg caccgaggcc ccagatcaga tcccatacaa
      181 tggggtacct tctgggcatc cttcagcccc ttgttgaata cgcttgagga gagccatttg
      241 actctttcca caactatcca actcacaacg tggcactggg gttgtgccgc ctttgcaggt
      301 gtatcttata cacgtggctt ttggccgcag aggcacctgt cgccaggtgg ggggttccgc
      361 tgcctgcaaa gggtcgctac agacgttgtt tgtcttcaag aagcttccag aggaactgct
      421 tccttcacga cattcaacag accttgcatt cctttggcga gaggggaaag acccctagga
      481 atgctcgtca agaagacagg gccaggtttc cgggccctca cattgccaaa agacggcaat
      541 atggtggaaa ataacatata gacaaacgca caccggcctt attccaagcg gcttcggcca
//
