LOCUS       sequence-80073-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains the Gateway selection cassette (ccdB ChlorR) flanked by 
            AttR1 and AttR3 sites for the creation of Destination vectors .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pBEG R1-ChlorR-R3   #BGV148
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..177
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(242..547)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(344..363)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cgactcacta tagggcgaat tggggatcga agttcaccgt taacgccact agccaactat
       61 gtataataaa gttgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat
      121 tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt cactatgggc
      181 cggggttggc gcgccgatat cgtcgacctg cagactggct gtgtataagg gagcctgaca
      241 tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga
      301 gatcagccac ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca
      361 tgcgccagct ttcatccccg atatgcacca ccgggtaaag ttcacggggg actttatctg
      421 acagcagacg tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat
      481 cactctgtac atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa
      541 actgcatttc accagcccct gttctcgtca gcaaaagagc cgttcatttc aataaaccgg
      601 gcgacctcag ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg
      661 gcttcattct gcatggttgt gcttaccaga ccggagatat tgacatcata tatgccttga
      721 gcaactgata gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcatacctct
      781 ttttgacata cttcgggtat acatatcagt atatattctt ataccgcaaa aatcagcgcg
      841 caaatacgca tactg
//
