LOCUS       sequence-80088-e         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre dependent expression of GCaMP6s Ca sensor and physically 
            separate nuclear localized dTomato fluorophore.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  TITLE     Ting GCaMP6 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(39..72)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(123..156)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(257..280)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(455..474)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgtcgtgagg taccggatcc tctagagtcg actccggaat aacttcgtat aggatacttt
       61 atacgaagtt atgcagaatg gtagctggat tgtagctgct attagcaata tgaaacctct
      121 taataacttc gtatagcata cattatacga agttatggcg cgccgtcgac ttacttgtac
      181 agctcgtcca tgccgtacag gaacaggtgg tggcggccct cggagcgctc gtactgttcc
      241 acgatggtgt agtcctcgtt gtgggaggtg atgtccagct tggtgtccac gtagtagtag
      301 ccgggcagtt gcacgggctt cttggccatg tagatggtct tgaactccac caggtagtgg
      361 ccgccgtcct tcagcttcag ggcctggtgg atctcgccct tcagcacgcc gtcgcggggg
      421 tacaggcgct cggtggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg
      481 ggggggaagt tggtgccgcg catcttcacc ttgtagatca gcgtgccgtc ctgcagggag
      541 gagtcctggg tcacggtcac cagaccgccg tcctcgaagt tcatcacgcg ctcccacttg
      601 aagccctcgg ggaaggacag cttcttgtaa tcggggatgt cggcggggtg cttcacgtac
      661 gccttggagc cgtacatgaa ctggggggac aggatgtccc aggcga
//
