LOCUS       sequence-80090-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Neuronal expression of GCaMP6f Ca sensor and physically separate 
            nuclear localized dTomato fluorophore .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  TITLE     Ting GCaMP6 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAV-hSyn1-GCaMP6f-P2A-nls-dTomato
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(73..771)
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(156..179)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(354..373)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     623..641
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(772..792)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(805..861)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattccct gcaggggcgc
       61 gccgtcgact tacttgtaca gctcgtccat gccgtacagg aacaggtggt ggcggccctc
      121 ggagcgctcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt
      181 ggtgtccacg tagtagtagc cgggcagttg cacgggcttc ttggccatgt agatggtctt
      241 gaactccacc aggtagtggc cgccgtcctt cagcttcagg gcctggtgga tctcgccctt
      301 cagcacgccg tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt
      361 ctgcattacg gggccgtcgg gggggaagtt ggtgccgcgc atcttcacct tgtagatcag
      421 cgtgccgtcc tgcagggagg agtcctgggt cacggtcacc agaccgccgt cctcgaagtt
      481 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcttgtaat cggggatgtc
      541 ggcggggtgc ttcacgtacg ccttggagcc gtacatgaac tggggggaca ggatgtccca
      601 ggcgaagggc agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta
      661 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcatgg agccctccat
      721 gcgcaccttg aagcgcatga actctttgat gacctcctcg cccttgctca ccaccttcct
      781 cttcttcttg ggggccattc tagatggccc gggattctct tcgacatccc ctgcttgttt
      841 caacagggag aagttagtgg ctccgcttcc gttatccttc gctgtcatca tttgtacaaa
      901 ctcttcgta
//
