LOCUS       sequence-80100-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Heo I, Joo C, Cho J, Ha M, Han J, Kim VN
  TITLE     Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA.
  JOURNAL   Mol Cell. 2008 Oct 24;32(2):276-84. doi: 
            10.1016/j.molcel.2008.09.014.
  PUBMED    18951094
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2008.09"; journalName: "Mol Cell"; date: 
            "2008-10-24- 24"; volume: "32"; issue: "2"; pages: "276-84"
COMMENT     Sequence Label: pcDNA3-FLAG-Lin28A
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..106
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(776..794)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(777..794)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(814..831)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctta tggactacaa agacgatgac gataaaggat ccatgggctc
      121 cgtgtccaac cagcagtttg caggtggctg cgccaaggcg gcagaagagg cgcccgagga
      181 ggcgccggag gacgcggccc gggcggcgga cgagcctcag ctgctgcacg gtgcgggcat
      241 ctgtaagtgg ttcaacgtgc gcatggggtt cggcttcctg tccatgaccg cccgcgccgg
      301 ggtcgcgctc gaccccccag tggatgtctt tgtgcaccag agtaagctgc acatggaagg
      361 gttccggagc ttgaaggagg gtgaggcagt ggagttcacc tttaagaagt cagccaaggg
      421 tctggaatcc atccgtgtca ccggacctgg tggagtattc tgtattggga gtgagaggcg
      481 gccaaaagga aagagcatgc agaagcgcag atcaaaagga gacaggtgct acaactgtgg
      541 aggtctagat catcatgcca aggaatgcaa gctgccaccc cagcccaaga agtgccactt
      601 ctgccagagc atcagccata tggtagcctc atgtccgctg aaggcccagc agggccctag
      661 tgcacaggga aagccaacct actttcgaga ggaagaagaa gaaatccaca gccctaccct
      721 gctcccggag gcacagaatt gagccacact cgagcatgca tctagagggc cctattctat
      781 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
      841 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
      901 tcctttccta ataaaatga
//
