LOCUS       sequence-80108-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Heo I, Joo C, Kim YK, Ha M, Yoon MJ, Cho J, Yeom KH, Han J, Kim VN
  TITLE     TUT4 in concert with Lin28 suppresses microRNA biogenesis through 
            pre-microRNA uridylation.
  JOURNAL   Cell. 2009 Aug 21;138(4):696-708. doi: 10.1016/j.cell.2009.08.002.
  PUBMED    19703396
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2009.08"; journalName: "Cell"; date: "2009-08-21- 
            21"; volume: "138"; issue: "4"; pages: "696-708"
COMMENT     Sequence Label: pcDNA3-V5-Lin28A
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             85..126
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        complement(796..814)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(797..814)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(834..851)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tatgggtaag cctatcccta accctctcct cggtctcgat
      121 tctacgggat ccatgggctc cgtgtccaac cagcagtttg caggtggctg cgccaaggcg
      181 gcagaagagg cgcccgagga ggcgccggag gacgcggccc gggcggcgga cgagcctcag
      241 ctgctgcacg gtgcgggcat ctgtaagtgg ttcaacgtgc gcatggggtt cggcttcctg
      301 tccatgaccg cccgcgccgg ggtcgcgctc gaccccccag tggatgtctt tgtgcaccag
      361 agtaagctgc acatggaagg gttccggagc ttgaaggagg gtgaggcagt ggagttcacc
      421 tttaagaagt cagccaaggg tctggaatcc atccgtgtca ccggacctgg tggagtattc
      481 tgtattggga gtgagaggcg gccaaaagga aagagcatgc agaagcgcag atcaaaagga
      541 gacaggtgct acaactgtgg aggtctagat catcatgcca aggaatgcaa gctgccaccc
      601 cagcccaaga agtgccactt ctgccagagc atcagccata tggtagcctc atgtccgctg
      661 aaggcccagc agggccctag tgcacaggga aagccaacct actttcgaga ggaagaagaa
      721 gaaatccaca gccctaccct gctcccggag gcacagaatt gagccacact cgagcatgca
      781 tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac
      841 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
      901 ggaag
//
