LOCUS       sequence-80112-e       12550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of synthetic 
            trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12550)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 12550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pMDC32B-AtTAS1c-B/c
FEATURES             Location/Qualifiers
     source          1..12550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             425..1219
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      1306..1894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1795..1814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2048..2065
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2080..2220
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2146..2165)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2306..2328
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2564..2758
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2824..3891)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(4325..4954)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6254..6278
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6466..6488
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6480..6497
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6481..6497
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6924..7268
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    7328..7352
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(7824..7829,7830..7847,7848..7854)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7908..8567
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7974..7993)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8909..9214
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9093..9112
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(9806..9830)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(10159..10175)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10159..10175)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10172..10194)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10183..10199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10207..10213,10214..10231,10232..10237))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10252..10273
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10464..11141
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             11209..12234
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    12274..12448
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12526..12550
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
       61 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
      121 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
      181 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
      241 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
      301 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
      361 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
      421 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
      481 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
      541 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
      601 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
      661 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
      721 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
      781 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
      841 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
      901 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
      961 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     1021 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     1081 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     1141 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     1201 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     1261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1441 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     2101 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2161 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     2221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2341 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     2401 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     2461 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     2521 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     2581 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     2641 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     2701 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     2761 aaagagaact tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     2821 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     2881 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     2941 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     3001 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     3061 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     3121 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     3181 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     3241 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     3301 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     3361 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     3421 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     3481 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     3541 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     3601 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     3661 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     3721 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     3781 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     3841 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgtcacgccg
     3901 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta
     3961 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
     4021 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
     4081 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
     4141 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
     4201 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
     4261 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
     4321 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
     4381 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
     4441 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
     4501 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
     4561 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
     4621 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
     4681 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
     4741 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
     4801 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
     4861 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
     4921 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
     4981 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
     5041 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
     5101 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
     5161 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
     5221 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
     5281 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
     5341 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
     5401 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
     5461 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
     5521 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
     5581 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
     5641 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
     5701 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
     5761 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
     5821 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
     5881 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
     5941 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
     6001 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
     6061 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
     6121 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
     6181 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc taataaacgc
     6241 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa
     6301 ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca ttcaggctgc
     6361 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     6421 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     6481 gtaaaacgac ggccagtgcc aagcttggcg tgcctgcagg tcaacatggt ggagcacgac
     6541 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
     6601 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
     6661 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
     6721 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
     6781 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     6841 tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca
     6901 gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc
     6961 ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt
     7021 ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc
     7081 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     7141 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     7201 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     7261 gagaggacct cgactctaga ggatccccgg gtaccgggcc ccccctcgag gcgcgccaag
     7321 ctatcaaaca agtttgtaca aaaaagcagg ctccgcggcc gcccccttca ccccttcacc
     7381 aaacctaaac ctaaacggct aagcccgacg tcaaatacca aaaagagaaa aacaagagcg
     7441 ccgtcaagct ctgcaaatac gatctgtaag tccatcttaa cacaaaagtg agatgggttc
     7501 ttagatcatg ttccgccgtt agatcgagtc atggtcttgt ctcatagaaa ggtactttcg
     7561 tttacttctt ttgagtatcg agtagagcgt cgtctatagt tagtttgaga ttgcgtttgt
     7621 cagaagttag gttcaatgtc ccggtccaat tttcaccagc catgtgtcag tttcgttcct
     7681 tcccgtcctc ttctttgatt tcgttgggtt acggatgttt tcgagatgaa acagcattgt
     7741 tttgttgtga tttttctcta caagcgaata gaccatttat cggtggatct tagaaaatta
     7801 agagaccatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga
     7861 ttttgagtta ggagccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
     7921 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     7981 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     8041 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     8101 gcctgatgaa tgctcatccg gagttccgta tggcaatgaa agacggtgag ctggtgatat
     8161 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     8221 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     8281 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     8341 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     8401 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     8461 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     8521 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggagccg
     8581 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
     8641 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat
     8701 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
     8761 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
     8821 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
     8881 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga
     8941 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccggccgacg
     9001 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta
     9061 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
     9121 gccggtttcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
     9181 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
     9241 gtctgcacct cgacggtctc agaactagaa aagacattgg acatattcca ggatatgcaa
     9301 aagaaaacaa tgaatattgt tttgaatgtg ttcaagtaaa tgagattttc aagtcgtcta
     9361 aagaacagtt gctaatacag ttacttattt caataaataa ttggttctaa taatacaaaa
     9421 catattcgag gatatgcaga aaaaaagatg tttgttattt tgaaaagctt gagtagtttc
     9481 tctccgaggt gtagcgaaga agcatcatct actttgtaat gtaattttct ttatgttttc
     9541 actttgtaat tttatttgtg ttaatgtacc atggccgata tcggttttat tgaaagaaaa
     9601 tttatgttac ttctgttttg gctttgcaat cagttatgct agttttctta taccctttcg
     9661 taagcttcct aaggaatcgt tcattgattt ccactgcttc attgtatatt aaaactttac
     9721 aactgtatcg accatcatat aattctgggt caagagatga aaatagaaca ccacatcgta
     9781 aagtgaaata agggtgggcg cgccgaccca gctttcttgt acaaagtggt tcgataattc
     9841 cttaattaac tagttctaga gcggccgccc accgcggtgg agctcgaatt tccccgatcg
     9901 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     9961 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
    10021 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
    10081 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
    10141 actgaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    10201 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    10261 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    10321 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctagag
    10381 cagcttgcca acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
    10441 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
    10501 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
    10561 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
    10621 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
    10681 ccaaccacgt cttcaaagca agtggattga tgtgataaca tggtggagca cgacactctc
    10741 gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt
    10801 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
    10861 atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga
    10921 aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg
    10981 aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
    11041 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agaccttcct
    11101 ctatataagg aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta
    11161 caaatctatc tctctcgagc tttcgcagat cccggggggc aatgagatat gaaaaagcct
    11221 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
    11281 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
    11341 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
    11401 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggagtttagc
    11461 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
    11521 gaaaccgaac tgcccgctgt tctacaaccg gtcgcggagg ctatggatgc gatcgctgcg
    11581 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
    11641 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
    11701 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
    11761 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
    11821 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
    11881 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
    11941 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccacg actccgggcg
    12001 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
    12061 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
    12121 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
    12181 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagaa atagagtaga
    12241 tgccgaccgg atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt
    12301 cccagataag ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa
    12361 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
    12421 accaaaatcc agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat
    12481 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata
    12541 tatcctgcca
//
