LOCUS       sequence-80115-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Lee Y, Hur I, Park SY, Kim YK, Suh MR, Kim VN
  TITLE     The role of PACT in the RNA silencing pathway.
  JOURNAL   EMBO J. 2006 Feb 8;25(3):522-32. Epub 2006 Jan 19.
  PUBMED    16424907
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2006-02-8"; volume: "25(3)"; pages: "522-32. Epub 2006 Jan 
            19"
COMMENT     Sequence Label: pcDNA3.1-FLAG-Dicer-delPAZ
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagccca ccatggacta caaggacgac gatgacaaga
      121 agctttaccc taacttagac ttcgggtgga aaaaatctat tgacagcaaa tctttcatct
      181 caatttctaa ctcctcttca gctgaaaatg ataattactg taagcacagc acaattgtcc
      241 ctgaaaatgc tgcacatcaa ggtgctaata gaacctcctc tctagaaaat catgaccaaa
      301 tgtctgtgaa ctgcagaacg ttgctcagcg agtcccctgg taagctccac gttgaagttt
      361 cagcagatct tacagcaatt aatggtcttt cttacaatca aaatctcgcc aatggcagtt
      421 atgatttagc taacagagac ttttgccaag gaaatcagct aaattactac aagcaggaaa
      481 tacccgtgca accaactacc tcatattcca ttcagaattt atacagttac gagaaccagc
      541 cccagcccag cgatgaatgt actctcctga gtaataaata ccttgatgga aatgctaaca
      601 aatctacctc agatggaagt cctgtgatgg ccgtaatgcc tggtacgaca gacactattc
      661 aagtgctcaa gggcaggatg gattctgagc agagcccttc tattgggtac tcctcaagga
      721 ctcttggccc caatcctgga cttattcttc aggctttgac tctgtcaaac gctagtgatg
      781 gatttaacct ggagcggctt gaaatgcttg gcgactcctt tttaaagcat gccatcacca
      841 catatctatt t
//
