LOCUS       sequence-80116-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Heo I, Joo C, Cho J, Ha M, Han J, Kim VN
  TITLE     Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA.
  JOURNAL   Mol Cell. 2008 Oct 24;32(2):276-84. doi: 
            10.1016/j.molcel.2008.09.014.
  PUBMED    18951094
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2008.09"; journalName: "Mol Cell"; date: 
            "2008-10-24- 24"; volume: "32"; issue: "2"; pages: "276-84"
COMMENT     Sequence Label: pcDNA3-pri-let-7b
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(355..373)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(356..373)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(393..410)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    399..623
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(756..775)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc gagctcggat cctgctctgg tgactgagga
      121 ccgccaggca ggggctggtg ctgggcgggg ggcggcgggc cctcccgcag tgcaaggccg
      181 ggcctggcgg ggtgaggtag taggttgtgt ggtttcaggg cagtgatgtt gcccctcgga
      241 agataactat acaacctact gccttccctg aggagcccag tgacacgacc ccatgggagg
      301 gccgccccct acctcagtga catgaccctc gagcatgcat ctagagggcc ctattctata
      361 gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
      421 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
      481 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
      541 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
      601 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
      661 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      721 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      781 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
      841 ccgatttagt gctttacggc acctc
//
