LOCUS       sequence-80117-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Heo I, Joo C, Cho J, Ha M, Han J, Kim VN
  TITLE     Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA.
  JOURNAL   Mol Cell. 2008 Oct 24;32(2):276-84. doi: 
            10.1016/j.molcel.2008.09.014.
  PUBMED    18951094
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2008.09"; journalName: "Mol Cell"; date: 
            "2008-10-24- 24"; volume: "32"; issue: "2"; pages: "276-84"
COMMENT     Sequence Label: pcDNA3-FLAG-Lin28B
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             85..108
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tatggactac aaagacgatg acgataaagg atccatggcc
      121 gaaggcgggg ctagcaaagg tggtggagaa gagcccggga agctgccgga gccggcagag
      181 gaggaatccc aggttttgcg cggaactggc cactgtaagt ggttcaatgt gcgcatggga
      241 tttggattca tctccatgat aaaccgagag ggaagcccct tggatattcc agtcgatgta
      301 tttgtacacc aaagcaaact attcatggaa ggatttagaa gcctaaaaga aggagaacca
      361 gtggaattca catttaaaaa atcttccaaa ggccttgagt caatacgggt aacaggacct
      421 ggtgggagcc cctgtttagg aagtgaaaga agacccaaag ggaagacact acagaaaaga
      481 aaaccaaagg gagatagatg ctacaactgt ggtggccttg atcatcatgc taaggaatgt
      541 agtctacctc ctcagccaaa gaagtgccat tactgtcaga gcatcatgca catggtggca
      601 aactgcccac ataaaaatgt tgcacagcca cccgcgagtt ctcagggaag acaggaagca
      661 gaatcccagc catgcacttc aactctccct cgagaagtgg gaggcgggca tggctgtaca
      721 tcaccaccgt ttcctcagga ggctagggca gagatctcag aacggtcagg caggtcacct
      781 caagaagctt cctccacgaa gtcatctata gcaccagaag agcaaagcaa a
//
