LOCUS       sequence-80118-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Heo I, Joo C, Kim YK, Ha M, Yoon MJ, Cho J, Yeom KH, Han J, Kim VN
  TITLE     TUT4 in concert with Lin28 suppresses microRNA biogenesis through 
            pre-microRNA uridylation.
  JOURNAL   Cell. 2009 Aug 21;138(4):696-708. doi: 10.1016/j.cell.2009.08.002.
  PUBMED    19703396
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2009.08"; journalName: "Cell"; date: "2009-08-21- 
            21"; volume: "138"; issue: "4"; pages: "696-708"
COMMENT     Sequence Label: pcDNA3-FLAG-Lin28B-mCCHC
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             85..108
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      590..599
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tatggactac aaagacgatg acgataaagg atccatggcc
      121 gaaggcgggg ctagcaaagg tggtggagaa gagcccggga agctgccgga gccggcagag
      181 gaggaatccc aggttttgcg cggaactggc cactgtaagt ggttcaatgt gcgcatggga
      241 tttggattca tctccatgat aaaccgagag ggaagcccct tggatattcc agtcgatgta
      301 tttgtacacc aaagcaaact attcatggaa ggatttagaa gcctaaaaga aggagaacca
      361 gtggaattca catttaaaaa atcttccaaa ggccttgagt caatacgggt aacaggacct
      421 ggtgggagcc cctgtttagg aagtgaaaga agacccaaag ggaagacact acagaaaaga
      481 aaaccaaagg gagatagatg ctacaactgt ggtggccttg atgctcatgc taaggaatgt
      541 agtctacctc ctcagccaaa gaagtgccat tactgtcaga gcatcatggc catggtggca
      601 aactgcccac ataaaaatgt tgcacagcca cccgcgagtt ctcagggaag acaggaagca
      661 gaatcccagc catgcacttc aactctccct cgagaagtgg gaggcgggca tggctgtaca
      721 tcaccaccgt ttcctcagga ggctagggca gagatctcag aacggtcagg caggtcacct
      781 caagaagctt cctccacgaa gtcatctata gcaccagaag agcaaagcaa aaaggggcct
      841 tcagtccaaa aaaggaaaaa gacataacag gtcttcttca tatgttcttt cctttacccg
      901 gttgcaa
//
