LOCUS       sequence-80122-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Heo I, Joo C, Cho J, Ha M, Han J, Kim VN
  TITLE     Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA.
  JOURNAL   Mol Cell. 2008 Oct 24;32(2):276-84. doi: 
            10.1016/j.molcel.2008.09.014.
  PUBMED    18951094
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2008.09"; journalName: "Mol Cell"; date: 
            "2008-10-24- 24"; volume: "32"; issue: "2"; pages: "276-84"
COMMENT     Sequence Label: pcDNA3-FLAG-Lin28A-mCCHC
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             85..108
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(778..796)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(779..796)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(816..833)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tatggactac aaagacgatg acgataaagg atccatgggc
      121 tccgtgtcca accagcagtt tgcaggtggc tgcgccaagg cggcagaaga ggcgcccgag
      181 gaggcgccgg aggacgcggc ccgggcggcg gacgagcctc agctgctgca cggtgcgggc
      241 atctgtaagt ggttcaacgt gcgcatgggg ttcggcttcc tgtccatgac cgcccgcgcc
      301 ggggtcgcgc tcgacccccc agtggatgtc tttgtgcacc agagtaagct gcacatggaa
      361 gggttccgga gcttgaagga gggtgaggca gtggagttca cctttaagaa gtcagccaag
      421 ggtctggaat ccatccgtgt caccggacct ggtggagtat tctgtattgg gagtgagagg
      481 cggccaaaag gaaagagcat gcagaagcgc agatcaaaag gagacaggtg ctacaactgt
      541 ggaggtctag atgctcatgc caaggaatgc aagctgccac cccagcccaa gaagtgccac
      601 ttctgccaga gcatcagcgc tatggtagcc tcatgtccgc tgaaggccca gcagggccct
      661 agtgcacagg gaaagccaac ctactttcga gaggaagaag aagaaatcca cagccctacc
      721 ctgctcccgg aggcacagaa ttgagccaca ctcgagcatg catctagagg gccctattct
      781 atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
      841 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaa
//
