LOCUS       sequence-80126-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Yeom KH, Lee Y, Han J, Suh MR, Kim VN
  TITLE     Characterization of DGCR8/Pasha, the essential cofactor for Drosha 
            in primary miRNA processing.
  JOURNAL   Nucleic Acids Res. 2006;34(16):4622-9. Epub 2006 Sep 8.
  PUBMED    16963499
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2006"; volume: "34(16)"; pages: "4622-9. Epub 
            2006 Sep 8"
COMMENT     Sequence Label: pcDNA3-V5-DGCR8-mDRBD2
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             86..127
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttatgggtaa gcctatccct aaccctctcc tcggtctcga
      121 ttctacggga tccactagta acggccgcca gtgtgctgga attcgattga atctgatgga
      181 gacagatgag agcccctctc cgctcccgtg tgggcccgca ggagaagcgg tgatggagag
      241 ccgagctcgc cccttccaag cgctgccccg tgagcagtct ccaccacctc ccctgcaaac
      301 gtccagtggt gcagaggtaa tggacgttgg ctctggtggt gatggacagt ccgaactccc
      361 tgctgaggac cccttcaact tctacggagc ttctcttctc tccaaaggat ccttctctaa
      421 gggccgcctc ctcatagacc cgaactgtag tggccacagc ccgcgcaccg cccggcacgc
      481 acctgcggtc cggaagttct cccctgacct taagttgctt aaggatgtaa agattagcgt
      541 gagctttacc gagagctgca ggagtaagga caggaaggtg ctgtacacag gagcagagcg
      601 cgacgtgcgg gcggagtgcg gtctgctcct tagccctgtc agtggggacg tgcatgcttg
      661 tccctttggc gggagtgttg gtgacggggt aggcataggg ggtgagagtg ctgataagaa
      721 ggatgaggag aatgagctgg atcaggaaaa gagagtggag tatgcagtgc tcgatgagtt
      781 agaagatttt actgacaatt tggagctaga tgaagaagga gcaggcgggt tcacggctaa
      841 agcaatcgtt cagagagaca gagtggatga agagg
//
