LOCUS       sequence-80128-e         305 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLSL with Zif268:FokI coding sequence followed by approximately 3kb 
            'filler' sequence, Rep is not included on this plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSLZ
FEATURES             Location/Qualifiers
     source          1..305
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..19
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8..30
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     27..43
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..43
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaattcgag
       61 ctcggtaccc ggggatcctc tagagtcgac ctgcagggag ggtcgtacga ataattcgta
      121 tccaacggaa atacctgata caatatacgc tccatcaaat accatcacat cgtatatgct
      181 tttatagtgt gaacaccttt aaccctagtg ggcgggaaat tttctacttt aaatctggac
      241 cgctcgtgct aaagcactcg cgataagggg gggccacgcc ggtaatatta aattcggcgt
      301 gggcc
//
