LOCUS       sequence-80130-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Yeom KH, Lee Y, Han J, Suh MR, Kim VN
  TITLE     Characterization of DGCR8/Pasha, the essential cofactor for Drosha 
            in primary miRNA processing.
  JOURNAL   Nucleic Acids Res. 2006;34(16):4622-9. Epub 2006 Sep 8.
  PUBMED    16963499
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2006"; volume: "34(16)"; pages: "4622-9. Epub 
            2006 Sep 8"
COMMENT     Sequence Label: pcDNA3-V5-DGCR8
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             85..126
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tatgggtaag cctatcccta accctctcct cggtctcgat
      121 tctacgggat ccactagtaa cggccgccag tgtgctggaa ttcgattgaa tctgatggag
      181 acagatgaga gcccctctcc gctcccgtgt gggcccgcag gagaagcggt gatggagagc
      241 cgagctcgcc ccttccaagc gctgccccgt gagcagtctc caccacctcc cctgcaaacg
      301 tccagtggtg cagaggtaat ggacgttggc tctggtggtg atggacagtc cgaactccct
      361 gctgaggacc ccttcaactt ctacggagct tctcttctct ccaaaggatc cttctctaag
      421 ggccgcctcc tcatagaccc gaactgtagt ggccacagcc cgcgcaccgc ccggcacgca
      481 cctgcggtcc ggaagttctc ccctgacctt aagttgctta aggatgtaaa gattagcgtg
      541 agctttaccg agagctgcag gagtaaggac aggaaggtgc tgtacacagg agcagagcgc
      601 gacgtgcggg cggagtgcgg tctgctcctt agccctgtca gtggggacgt gcatgcttgt
      661 ccctttggcg ggagtgttgg tgacggggta ggcatagggg gtgagagtgc tgataagaag
      721 gatgaggaga atgagctgga tcaggaaaag agagtggagt atgcagtgct cgatgagtta
      781 gaagatttta ctgacaattt ggagctagat gaagaaggag caggcgggtt cacggctaaa
      841 gcaatcgttc agagagacag agtggatgaa gaggccttga atttccccta cgaggatgac
      901 tttgacaacg atgtggatgc tctgctggaa
//
