LOCUS       sequence-80133-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Yeom KH, Lee Y, Han J, Suh MR, Kim VN
  TITLE     Characterization of DGCR8/Pasha, the essential cofactor for Drosha 
            in primary miRNA processing.
  JOURNAL   Nucleic Acids Res. 2006;34(16):4622-9. Epub 2006 Sep 8.
  PUBMED    16963499
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2006"; volume: "34(16)"; pages: "4622-9. Epub 
            2006 Sep 8"
COMMENT     Sequence Label: pcDNA3-V5-DGCR8-mDRBD1
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             88..129
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttatgggt aagcctatcc ctaaccctct cctcggtctc
      121 gattctacgg gatccactag taacggccgc cagtgtgctg gaattcgatt gaatctgatg
      181 gagacagatg agagcccctc tccgctcccg tgtgggcccg caggagaagc ggtgatggag
      241 agccgagctc gccccttcca agcgctgccc cgtgagcagt ctccaccacc tcccctgcaa
      301 acgtccagtg gtgcagaggt aatggacgtt ggctctggtg gtgatggaca gtccgaactc
      361 cctgctgagg accccttcaa cttctacgga gcttctcttc tctccaaagg atccttctct
      421 aagggccgcc tcctcataga cccgaactgt agtggccaca gcccgcgcac cgcccggcac
      481 gcacctgcgg tccggaagtt ctcccctgac cttaagttgc ttaaggatgt aaagattagc
      541 gtgagcttta ccgagagctg caggagtaag gacaggaagg tgctgtacac aggagcagag
      601 cgcgacgtgc gggcggagtg cggtctgctc cttagccctg tcagtgggga cgtgcatgct
      661 tgtccctttg gcgggagtgt tggtgacggg gtaggcatag ggggtgagag tgctgataag
      721 aaggatgagg agaatgagct ggatcaggaa aagagagtgg agtatgcagt gctcgatgag
      781 ttagaagatt ttactgacaa tttggagcta gatgaagaa
//
