LOCUS       sequence-80143-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains the effective Fh8 fusion tag enhancing the solubility of 
            the target recombinant protein and increases its expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Costa SJ, Almeida A, Castro A, Domingues L, Besir H
  TITLE     The novel Fh8 and H fusion partners for soluble protein expression 
            in Escherichia coli: a comparison with the traditional gene fusion 
            technology.
  JOURNAL   Appl Microbiol Biotechnol. 2013 Aug;97(15):6779-91. doi: 
            10.1007/s00253-012-4559-1. Epub 2012 Nov 20.
  PUBMED    23160981
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-012-4559-1"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2013-08"; volume: "97"; issue: "15"; pages: 
            "6779-91"
COMMENT     Sequence Label: pETFH8
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    79..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             118..140
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             153..170
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             384..404
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      406..415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             834..851
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
       61 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
      121 gtttaacttt aagaaggaga tataccatga aacatcacca tcaccatcac cccatgtccc
      181 ctagtgttca agaggttgaa aaactccttc atgttctcga tcgcaacggt gacgggaagg
      241 tttctgccga ggagttgaaa gccttcgctg atgattcaaa atgtcctctg gactccaata
      301 agatcaaggc tttcattaag gaacacgata aaaacaagga tggcaagctt gatttgaaag
      361 aactcgtttc gattttgtca tcagagaatc tttattttca gggctccatg gcggcttatg
      421 tgtatcgctc agcgttcagt gtggggctgg agacccgcgt cactgttccc aatgtaccca
      481 ttcgctttac taagatcttc tacaaccaac agaatcatta tgacggcagc actggcaagt
      541 tctattgcaa cattccggga ctctactact tctcttacca catcacggtg tacatgaaag
      601 atgtgaaggt gagcctcttc aagaaggaca aggccgttct cttcacctac gaccagtatc
      661 aggaaaagaa tgtggaccag gcctctggct ctgtgctcct ccatctggag gtgggagacc
      721 aagtctggct ccaggtgtat ggggatgggg accacaatgg actctatgca gataacgtca
      781 acgactctac atttactggc tttcttctct accatgatac caactgactc gagcaccacc
      841 accaccacca ctgagatccg gctgctaaca aag
//
