LOCUS       sequence-80146-e         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLSL with GFP followed by the us:NPTII repair template, Rep is not 
            included on this plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSLGFP
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     100..128
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(246..266)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(273..338)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(360..384)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agttttccag tagtgcaaat aaatttaagg gtaagttttc cgtatgttgc atcaccttca
       61 ccctctccac tgacagaaaa tttgtgccca ttaacatcac catctaattc aacaagaatt
      121 gggacaactc cagtgaaaag ttcttctcct ttactgaatt cggccgagga taatgatagg
      181 agaagtgaaa agatgagaaa gagaaaaaga ttagtcttca ttctagaggg aacgccatgg
      241 ataccgactt tgcgcttttt cttaggagcc attttatcgt catcgtcttt gtagtcaata
      301 tcgtggtcct tgtagtcgcc atcgtgatcc ttataatcca tggtgaaggg cgaattcgga
      361 gcctgctttt ttgtacaaac ttgtagaaat gatttaaatt gctgcaatag aagtagaatg
      421 cttgattgct tgagattcgt ttgttttgta tatgttgtgt tgagaactct cgacgtcctg
      481 caaacaatac acaacaaaga caatgagatt tggttttata aacaatcaag aatcttaggg
      541 agggtcgtac gaataattcg tatccaacgg aaatacctga tacaatatac gctccatcaa
      601 ataccatcac atcgtatatg cttttatagt gtgaacacct ttaaccctag tgggcgggaa
      661 attttctact ttaaatctgg accgctcgtg ctaaagcact cg
//
