LOCUS       sequence-80153-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pMDC32 with Zif268:FokI followed by approximately 3kb of filler 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: p35SZ
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        404..748
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     692..712
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    808..832
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             866..886
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcaacatggt ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag
       61 aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat
      121 tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct
      181 acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg
      241 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
      301 cgtcttcaaa gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact
      361 ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa
      421 gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga
      481 agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca
      541 tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
      601 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
      661 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
      721 aaggaagttc atttcatttg gagaggacct cgactctaga ggatccccgg gtaccgggcc
      781 ccccctcgag gcgcgccaag ctatcaaaca agtttgtaca aaaaagcagg ctccgaattc
      841 gcccttcacc atggcttcct cccctccaaa gaaaaagaga aaggttcttg aattgcctta
      901 cgcttgtcca gttgagtctt gtgatagacg ttttagtagg tcagacgaac tc
//
