LOCUS       sequence-80156-e        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for CONK1-lumitoxin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Schmidt D, Tillberg PW, Chen F, Boyden ES
  TITLE     A fully genetically encoded protein architecture for optical control
            of peptide ligand concentration.
  JOURNAL   Nat Commun. 2014 Jan 10;5:3019. doi: 10.1038/ncomms4019.
  PUBMED    24407101
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-01-10"; volume: "5:3019. doi"; pages: " 
            10.1038/ncomms4019"
COMMENT     Sequence Label: pcDNA3.1-CONK1-lumitoxin
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             156..170
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             375..398
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             438..866
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
     CDS             888..1034
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcat gagcgccctg ctgatcctgg
      121 ccctggtggg cgccgccgtg gccgactaca aggacgacga cgacgacaag ctgaagctta
      181 aggaccggcc cagcctgtgc gacctgcctg ccgattctgg ctctggcacc aaggccgaga
      241 agcggatcta ctacaacagc gcccggaagc agtgcctgag attcgactac acaggccagg
      301 gcggcaacga gaacaacttc cggcggacct acgactgcca gagaacctgc ctgtacaccg
      361 gtaccgccgc cgccgactac aaggacgacg acgacaagat cgacgccgcc gccggcggcg
      421 ccctgtgcaa cgaattcttg gctactacac ttgaacgtat tgagaagaac tttgtcatta
      481 ctgacccaag attgccagat aatcccatta tattcgcgtc cgatagtttc ttgcagttga
      541 cagaatatag ccgtgaagaa attttgggaa gaaactgcag gtttctacaa ggtcctgaaa
      601 ctgatcgcgc gacagtgaga aaaattagag atgccataga taaccaaaca gaggtcactg
      661 ttcagctgat taattataca aagagtggta aaaagttctg gaacctcttt cacttgcagc
      721 ctatgcgaga tcagaaggga gatgtccagt actttattgg ggttcagttg gatggaactg
      781 agcatgtccg agatgctgcc gagagagagg gagtcatgct gattaagaaa actgcagaaa
      841 atattgatga ggcggcaaaa gaacttagcg gccgcggtac tcgagttgct gtgggccagg
      901 acacgcagga ggtcatcgtg gtgccacact ccttgccctt taaggtggtg gtgatctcag
      961 ccatcctggc cctggtggtg ctcaccatca tctcccttat catcctcatc atgctttggc
     1021 agaagaaacc acgtaggatt cgtatggtga gcaagggcga ggaggataac atggccatca
     1081 t
//
