LOCUS       sequence-80158-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for CONK1-lumitoxin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Schmidt D, Tillberg PW, Chen F, Boyden ES
  TITLE     A fully genetically encoded protein architecture for optical control
            of peptide ligand concentration.
  JOURNAL   Nat Commun. 2014 Jan 10;5:3019. doi: 10.1038/ncomms4019.
  PUBMED    24407101
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-01-10"; volume: "5:3019. doi"; pages: " 
            10.1038/ncomms4019"
COMMENT     Sequence Label: pcDNA3.1-CONK1-lumitoxin
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(139..285)
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             complement(307..735)
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc
       61 catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt
      121 gctcaccata cgaatcctac gtggtttctt ctgccaaagc atgatgagga tgataaggga
      181 gatgatggtg agcaccacca gggccaggat ggctgagatc accaccacct taaagggcaa
      241 ggagtgtggc accacgatga cctcctgcgt gtcctggccc acagcaactc gagtaccgcg
      301 gccgctaagt tcttttgccg cctcatcaat attttctgca gttttcttaa tcagcatgac
      361 tccctctctc tcggcagcat ctcggacatg ctcagttcca tccaactgaa ccccaataaa
      421 gtactggaca tctcccttct gatctcgcat aggctgcaag tgaaagaggt tccagaactt
      481 tttaccactc tttgtataat taatcagctg aacagtgacc tctgtttggt tatctatggc
      541 atctctaatt tttctcactg tcgcgcgatc agtttcagga ccttgtagaa acctgcagtt
      601 tcttcccaaa atttcttcac ggctatattc tgtcaactgc aagaaactat cggacgcgaa
      661 tataatggga ttatctggca atcttgggtc agtaatgaca aagttcttct caatacgttc
      721 aagtgtagta gccaagaatt cgttgcacag ggcgccgccg g
//
