LOCUS       sequence-80161-e       14287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV driven mammalian expression of ligase-dead UBR5 with an 
            N-termianl GFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14287)
  AUTHORS   Gudjonsson T, Altmeyer M, Savic V, Toledo L, Dinant C, Grofte M, 
            Bartkova J, Poulsen M, Oka Y, Bekker-Jensen S, Mailand N, Neumann B,
            Heriche JK, Shearer R, Saunders D, Bartek J, Lukas J, Lukas C
  TITLE     TRIP12 and UBR5 suppress spreading of chromatin ubiquitylation at 
            damaged chromosomes.
  JOURNAL   Cell. 2012 Aug 17;150(4):697-709. doi: 10.1016/j.cell.2012.06.039. 
            Epub 2012 Aug 9.
  PUBMED    22884692
REFERENCE   2  (bases 1 to 14287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.06.039"; journalName: "Cell"; date: 
            "2012-08-17- 17"; volume: "150"; issue: "4"; pages: "697-709"
COMMENT     Sequence Label: GFP-UBR5 ∆HECT
FEATURES             Location/Qualifiers
     source          1..14287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        105..484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        485..688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     639..659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     685..709
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(813..815,816..818,819..1529)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(858..879)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1119..1138)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1466..1487
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1545..1569
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9984..10008)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10016..10057
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(10097..10116)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10141..10189
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10391..10817
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10478..10497)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10686..10707
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10826..10846)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10831..11160
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11011..11146
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11073..11092
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11208..11255
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11274..11672
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    11830..11951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11867..11886)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11921..11940
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12000..12016)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12000..12016)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12013..12035)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12024..12040
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12048..12054,12055..12072,12073..12078))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12093..12114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12231..12248)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12402..12990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12482..12501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13161..13952,13953..14021))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13784..13803
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14022..14126)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(14201..14220)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
       61 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      661 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      721 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      781 agaggatccc taccggtgat atcctcgaga ccatggtgag caagggcgag gagctgttca
      841 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      901 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      961 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcacc tacggcgtgc
     1021 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     1081 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     1141 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     1201 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     1261 aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc
     1321 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1381 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     1441 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1501 tcactctcgg catggacgag ctgtacaagg gctcgagccc atcaacaagt ttgtacaaaa
     1561 aagcaggctt ccggaccatg acgtccatcc atttcgtggt tcacccgctg ccgggcaccg
     1621 aggaccagct caatgacagg ttacgagaag tttctgagaa gctgaacaaa tataatttaa
     1681 acagccaccc ccctttgaat gtattggaac aggctactat taaacagtgt gtggtgggac
     1741 caaatcatgc tgcctttctt cttgaggatg gtagagtttg caggattggt ttttcagtac
     1801 agccagacag attggaattg ggtaaacctg ataataatga tgggtcaaag ttgaacagca
     1861 actcgggggc agggaggacg tcaaggcctg gtaggacaag cgactctcca tggtttctct
     1921 caggttctga gactctaggc aggctggcag gcaacacctt aggaagccgc tggagttctg
     1981 gagtgggtgg aagtggtgga ggatcctctg gtaggtcatc agctggagct cgagattccc
     2041 gccggcagac tcgagttatt cggacaggac gggatcgagg gtctgggctt ttgggcagtc
     2101 agccccagcc agttattcca gcatctgtca ttccagagga gctgatttca caggcccaag
     2161 ttgttttaca aggcaaatcc agaagtgtca ttattcgaga acttcagaga acaaatcttg
     2221 atgtgaacct tgctgtaaat aatttactta gccgggatga tgaagatgga gatgatgggg
     2281 atgatacagc cagcgaatct tatttgcctg gagaggatct tatgtctctc cttgatgccg
     2341 acattcattc tgcccaccca agtgtcatta ttgatgcaga tgccatgttt tctgaagaca
     2401 ttagctattt tggttaccct tcttttcgtc gttcatcact ttccaggcta ggctcatctc
     2461 gagttctcct tcttccctta gagagagact ctgagctgtt gcgtgaacgt gaatccgttt
     2521 tacgtttacg tgaacgaagg tggcttgatg gagcctcatt tgataatgaa aggggttcta
     2581 ccagcaagga aggagagcca aacttggata agaagaatac acctgttcaa agtccagtat
     2641 ctctaggaga agatttgcag tggtggcctg ataaggatgg aacaaaattc atctgtattg
     2701 gggctctgta ttctgaactt ctggctgtca gcagtaaagg agaactttat cagtggaaat
     2761 ggagtgaatc tgagccttac agaaatgccc agaatccttc attacatcat ccacgagcaa
     2821 catttttggg gttaaccaat gaaaagatag tcctcctgtc tgcaaatagc ataagagcaa
     2881 ctgtagctac agaaaataac aaggttgcta catgggtgga tgaaacttta agttctgtgg
     2941 cttctaaatt agagcacact gctcagactt actctgaact tcaaggagag cggatagttt
     3001 ctttacattg ctgtgccctt tacacctgcg ctcagctgga aaacagttta tattggtggg
     3061 gtgtagttcc ttttagtcaa aggaagaaaa tgttagagaa agctagagca aaaaataaaa
     3121 agcctaaatc cagtgctggt atttcttcaa tgccgaacat cactgttggt acccaggtat
     3181 gcttgagaaa taatcctctt tatcatgctg gagcagttgc attttcaatt agtgctggga
     3241 ttcctaaagt tggtgtctta atggagtcag tttggaatat gaatgacagc tgtagatttc
     3301 aacttagatc tcctgaaagc ttgaaaaaca tggaaaaagc tagcaaaact actgaagcta
     3361 agcctgaaag taagcaggag ccagtgaaaa cagaaatggg tcctccacca tctccagcat
     3421 ccacgtgtag tgatgcatcc tcaattgcca gcagtgcatc aatgccatac aaacgacgac
     3481 ggtcaacccc tgcaccaaaa gaagaggaaa aggtgaatga agagcagtgg tctcttcggg
     3541 aagtggtttt tgtggaagat gtcaagaatg ttcctgttgg caaggtgcta aaagtagatg
     3601 gtgcctatgt tgctgtaaaa tttccaggaa cctccagtaa tactaactgt cagaacagct
     3661 ctggtccaga tgctgaccct tcttctctcc tgcaggattg taggttactt agaattgatg
     3721 aattgcaggt tgtcaaaact ggtggaacac cgaaggttcc cgactgtttc caaaggactc
     3781 ctaaaaagct ttgtatacct gaaaaaacag aaatattagc agtgaatgta gattccaaag
     3841 gtgttcatgc tgttctgaag actggaaatt gggtgcgata ctgtatcttt gatcttgcta
     3901 caggaaaagc agaacaggaa aataattttc ctacaagcag cattgctttc cttggtcaga
     3961 atgagaggaa tgtagccatt ttcactgctg gacaggaatc tcccattatt cttcgagatg
     4021 gaaatggtac catctaccca atggccaaag attgcatggg aggaataagg gatcccgatt
     4081 ggctggatct tccacctatt agtagtcttg gaatgggtgt gcattcttta ataaatcttc
     4141 ctgccaattc aacaatcaaa aagaaagctg ctgttatcat catggctgta gagaaacaaa
     4201 ccttaatgca acacattctg cgctgtgact atgaggcctg tcgacaatat ctaatgaatc
     4261 ttgagcaagc ggttgtttta gagcagaatc tacagatgct gcagacattc atcagccaca
     4321 gatgtgatgg aaatcgaaat attttgcatg cttgtgtatc agtttgcttt ccaaccagca
     4381 ataaagaaac taaagaagaa gaggaagcgg agcgttctga aagaaataca tttgcagaaa
     4441 ggctttctgc tgttgaggcc attgcaaatg caatatcagt tgtttcaagt aatggcccag
     4501 gtaatcgggc tggatcatca agtagccgaa gtttgagatt acgggaaatg atgagacgtt
     4561 cgttgagagc agctggtttg ggtagacatg aagctggagc ttcatccagt gaccaccagg
     4621 atccagtttc accccccata gctcccccta gttgggttcc tgaccctcct gcgatggatc
     4681 ctgatggtga cattgatttt atcctggccc ccgctgtggg atctcttacc acagcagcaa
     4741 ccggtactgg tcaaggacca agcacctcca ctattccagg tccttccaca gagccatctg
     4801 tagtagaatc caaggatcga aaggcgaatg ctcattttat attgaaattg ttatgtgaca
     4861 gtgtggttct ccagccctat ctacgagaac ttctttctgc caaggatgca agagggatga
     4921 ccccatttat gtcagctgta agtggccgag cttatcctgc tgcaattacc atcttagaaa
     4981 ctgctcagaa aattgcaaaa gctgaaatat cctcaagtga aaaagaggaa gatgtattca
     5041 tgggaatggt ttgcccatca ggtaccaacc ctgatgactc tcctttatat gttttatgtt
     5101 gtaatgacac ttgcagtttt acatggactg gagcagagca cattaaccag gatatttttg
     5161 agtgtcgaac ttgtggcttg ctggagtcac tgtgttgttg tacggaatgt gcaagggttt
     5221 gtcataaagg tcatgattgc aaactcaaac ggacatcacc aacagcctac tgtgattgtt
     5281 gggagaaatg taaatgtaaa actcttattg ctggacagaa atctgctcgt cttgatctac
     5341 tttatcgcct gctcactgct actaatctgg ttactctgcc aaacagcagg ggagagcacc
     5401 tcttactatt cttagtacag acagtcgcaa ggcagacggt ggagcattgt caatacaggc
     5461 cacctcgaat cagggaagat cgtaaccgaa aaacagccag tcctgaagat tcagatatgc
     5521 cagatcatga tttagagcct ccaagatttg cccagcttgc attggagcgt gttctacagg
     5581 actggaatgc cttgaaatct atgattatgt ttgggtcgca ggagaataaa gaccctctta
     5641 gtgccagcag tagaataggc catcttttgc cagaagagca agtatacctc aatcagcaaa
     5701 gtggcacaat tcggctggac tgtttcactc attgccttat agttaagtgt acagcagata
     5761 ttttgctttt agatactcta ctaggtacac tagtgaaaga actccaaaac aaatatacac
     5821 ctggacgtag agaagaagct attgctgtga caatgaggtt tctacgttca gtggcaagag
     5881 tttttgttat tctgagtgtg gaaatggctt catccaaaaa gaaaaacaac tttattccac
     5941 agccaattgg aaaatgcaag cgtgtattcc aagcattgct accttacgct gtggaagaat
     6001 tgtgcaacgt agcagagtca ctgattgttc ctgtcagaat ggggattgct cgtccaactg
     6061 caccatttac cctggctagt actagcatag atgccatgca gggcagtgaa gaattatttt
     6121 cagtggaacc actaccacca cgaccatcat ctgatcagtc tagcagctcc agtcagtctc
     6181 agtcatccta catcatcagg aatccacagc agaggcgcat cagccagtca cagcccgttc
     6241 ggggcagaga tgaagaacag gatgatattg tttcagcaga tgtggaagag gttgaggtgg
     6301 tggagggtgt ggctggagaa gaggatcatc atgatgaaca ggaagaacac ggggaagaaa
     6361 atgctgaggc agagggacaa catgatgagc atgatgaaga cgggagtgat atggagctgg
     6421 acttgttagc agcagctgaa acagaaagtg atagtgaaag taaccacagc aaccaagata
     6481 atgctagtgg gcgcagaagc gttgtcactg cagcaactgc tggttcagaa gcaggagcaa
     6541 gcagtgttcc tgccttcttt tctgaagatg attctcaatc gaatgactca agtgattctg
     6601 atagcagtag tagtcagagt gacgacatag aacaggagac ctttatgctt gatgagccat
     6661 tagaaagaac cacaaatagc tcccatgcca atggtgctgc ccaagctccc cgttcaatgc
     6721 agtgggctgt ccgcaacacc cagcatcagc gagcagccag tacagcccct tccagtacat
     6781 ctacaccagc agcaagttca gcgggtttga tttatattga tccttcaaac ttacgccgga
     6841 gtggtaccat cagtacaagt gctgcagctg cagcagctgc tttggaagct agcaacgcca
     6901 gcagttacct aacatctgca agcagtttag ccagggctta cagcattgtc attagacaaa
     6961 tctcggactt gatgggcctt attcctaagt ataatcacct agtatactct cagattccag
     7021 cagctgtgaa attgacttac caagatgcag taaacttaca gaactatgta gaagaaaagc
     7081 ttattcccac ttggaactgg atggtcagta ttatggattc tactgaagct caattacgtt
     7141 atggttctgc attagcatct gctggtgatc ctggacatcc aaatcatcct cttcacgctt
     7201 ctcagaattc agcgagaaga gagaggatga ctgcgcgaga agaagctagc ttacgaacac
     7261 ttgaaggcag acgacgtgcc accttgctta gcgcccgtca aggaatgatg tctgcacgag
     7321 gagacttcct aaattatgct ctgtctctaa tgcggtctca taatgatgag cattctgatg
     7381 ttcttccagt tttggatgtt tgctcattga agcatgtggc atatgttttt caagcactta
     7441 tatactggat taaggcaatg aatcagcaga caacattgga tacacctcaa ctagaacgca
     7501 aaaggacgcg agaactcttg gaactgggta ttgataatga agattcagaa catgaaaatg
     7561 atgatgacac caatcaaagt gctactttga atgataagga tgatgactct cttcctgcag
     7621 aaactggcca aaaccatcca tttttccgac gttcagactc catgacattc cttgggtgta
     7681 tacccccaaa tccatttgaa gtgcctctgg ctgaagccat ccccttggct gatcagccac
     7741 atctgttgca gccaaatgct agaaaggagg atctttttgg ccgtccaagt cagggtcttt
     7801 attcttcatc tgccagtagt gggaaatgtt taatggaggt tacagtggat agaaactgcc
     7861 tagaggttct tccaacaaaa atgtcttatg ctgccaatct gaaaaatgta atgaacatgc
     7921 aaaaccggca aaaaaaagaa ggggaagaac agcccgtgct gccagaagaa actgagagtt
     7981 caaaaccagg gccatctgct catgatcttg ctgcacaatt aaaaagtagc ttactagcag
     8041 aaataggact tactgaaagt gaagggccac ctctcacatc tttcaggcca cagtgtagct
     8101 ttatgggaat ggttatttcc catgatatgc tgctaggacg ttggcgcctt tctttagaac
     8161 tgttcggcag ggtattcatg gaagatgttg gagcagaacc tggatcaatc ctaactgaat
     8221 tgggtggttt tgaggtaaaa gaatcaaaat tccgcagaga aatggaaaaa ctgagaaacc
     8281 agcagtcaag agatttgtca ctagaggttg atcgggatcg agatcttctc attcagcaga
     8341 ctatgaggca gcttaacaat cactttggtc gaagatgtgc tactacacca atggctgtac
     8401 acagagtaaa agtcacattt aaggatgagc caggagaggg cagtggtgta gcacgaagtt
     8461 tttatacagc cattgcacaa gcatttttat caaatgaaaa attgccaaat ctagagtgta
     8521 tccaaaatgc caacaaaggc acccacacaa gtttaatgca gagattaagg aaccgaggag
     8581 agagagaccg ggaaagggag agagaaaggg aaatgaggag gagtagtggt ttgcgagcag
     8641 gttctcggag ggaccgggat agagacttta gaagacagct ttccatcgac actaggccct
     8701 ttagaccagc ctctgaaggg aatcctagcg atgatcctga gcctttgcca gcacatcggc
     8761 aggcacttgg agagaggctt tatcctcgtg tacaagcaat gcaaccagca tttgcaagta
     8821 aaatcactgg catgttgttg gaattatccc cagctcagct gcttctcctt ctagcaagtg
     8881 aggattctct gagagcaaga gtggatgagg ccatggaact cattattgca catggacggg
     8941 aaaatggagc tgatagtatc ctggatcttg gattagtaga ctcctcagaa aaggtacagc
     9001 aggaaaaccg aaagcgccat ggctctagtc gaagtgtagt agatatggat ttagatgata
     9061 cagatgatgg tgatgacaat gcccctttgt tttaccaacc tgggaaaaga ggattttata
     9121 ctccaaggcc tggcaagaac acagaagcaa ggttgaattg tttcagaaac attggcagga
     9181 ttcttggact atgtctgtta cagaatgaac tctgtcctat cacattgaat agacatgtaa
     9241 ttaaagtatt gcttggtaga aaagtcaatt ggcatgattt tgcttttttt gatcctgtaa
     9301 tgtatgagag tttgcggcaa ctaatcctcg cgtctcagag ttcagatgct gatgctgttt
     9361 tctcagcaat ggatttggca tttgcaattg acctgtgtaa agaagaaggt ggaggacagg
     9421 ttgaactcat tcctaatggt gtaaatatac cagtcactcc acagaatgta tatgagtatg
     9481 tgcggaaata cgcagaacac agaatgttgg tagttgcaga acagccctta catgcaatga
     9541 ggaaaggtct actagatgtg cttccaaaaa attcattaga agatttaacg gcagaagatt
     9601 ttaggctttt ggtaaatggc tgcggtgaag tcaatgtgca aatgctgatc agttttacct
     9661 ctttcaatga tgaatcagga gaaaatgctg agaagcttct gcagttcaag cgttggttct
     9721 ggtcaatagt agagaagatg agcatgacag aacgacaaga tcttgtttac ttttggacat
     9781 caagcccatc actgccagcc agtgaagaag gattccagcc tatgccctca atcacaataa
     9841 gaccaccaga tgaccaacat cttcctactg caaatactgc catttctcga ctttacgtcc
     9901 cactctattc ctctaaacag attctcaaac agaaattgtt actcgccatt aagaccaaga
     9961 attttggttt tgtgtagccg cggacccagc tttcttgtac aaagtggttg ataacgggaa
    10021 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagttta
    10081 aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
    10141 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg
    10201 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
    10261 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
    10321 gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggctctagg
    10381 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
    10441 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
    10501 tttctcgcca cgttcgccgg ctttcccgtc aagctctaaa tcgggggctc cctttagggt
    10561 tccgatttag tgctttacgg cacctcgacc ccaaaaactt gattagggtg atggttcacg
    10621 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    10681 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    10741 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
    10801 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
    10861 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
    10921 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    10981 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
    11041 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
    11101 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
    11161 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
    11221 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
    11281 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca
    11341 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct
    11401 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg
    11461 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga
    11521 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg
    11581 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat
    11641 tgctgccctc tggttatgtg tgggagggct aagcacttcg tggccgagga gcaggactga
    11701 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
    11761 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
    11821 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    11881 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    11941 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
    12001 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
    12061 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    12121 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    12181 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
    12241 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    12301 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    12361 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    12421 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    12481 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    12541 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    12601 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    12661 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    12721 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    12781 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    12841 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    12901 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    12961 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    13021 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    13081 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    13141 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    13201 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    13261 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    13321 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    13381 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    13441 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    13501 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    13561 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    13621 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    13681 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    13741 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    13801 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    13861 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    13921 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    13981 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    14041 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    14101 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
    14161 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
    14221 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
    14281 gcaaaat
//
