LOCUS       sequence-80164-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-orfL6
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             181..204
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
      181 ctggaagttc tgttccaggg gccccatatg tgtccgctgc cgattgaact gaccgatcgt
      241 agcgttaatc gttggcctgc aagccagagc ggtgttagcc cgagccgtga tgcagcaaaa
      301 ctgccgacca ttctgggtcg tatgccggtt gcaaatcgta cccagcagcg tggtgaaaaa
      361 agccgtgaag aaattctgga tgcagcaagc cgtctgatgg gtgaacgtgg ttatgatggc
      421 accagcattg cagcagttag cgaagcagtt ggtattccga aaagcctggt gtttcatcat
      481 tttggtagca aaattggtct gctgaccgca gttatgaccc gtggtgcata tcgttttttt
      541 gaagcaatgg aagcagcaca ggcagatagt ccggaaaccg gcacccatct ggaacgtctg
      601 acctggtttc tgacaccggt tccgagcctg gcatgtctgg atagccatca gcctgcagca
      661 cgtaattatg caggtagcat gagcaatacc cgtagcacca caccgcgtcg tggtgttgca
      721 gttgaaacca ccgccagctt tccgaatccg gcacaggatt attttgatgg tcatctgagc
      781 ctggatcgtc atctgattcc gcgtccgctg agcacctttg ttctgcgtgt taccggtgat
      841 gcactggcaa gcatgggtat tcatgatggt gatgaactgc tggttgatcg tgtta
//
