LOCUS       sequence-80176-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-JadR2
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    43..67
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             82..104
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             123..140
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             150..173
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             795..812
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa
       61 caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca
      121 gccatcatca tcatcatcac agcagcggcc tggaagttct gttccagggg ccccatatga
      181 ccaaacaaga acgtgcaacc cgtacccgtg atgcactgat taaaagcgca gcacgtgaat
      241 ttgatgaaca tggttatgca ctggcaaaac tgagcgcaat tagcagcggt gccggtgtta
      301 gtccgggtgc actgcatttt cattttgaaa ataaagttgc cgcagccgtt gaaattgatg
      361 caagcaccac cctgcgtcgt accgcacgta ttgtttatca tcagcgtagc aatgcactgc
      421 agaatctggc agataccacc catgcactgg cacgtctggt tcgtgaagat gttgttgttc
      481 gtgcaggttt tcgtctgagc tgtagccagc tgtgtggcac cgatctgaat ctgcgtcaag
      541 aatggcagag ctgtgttcag cagcgtctgg ccgaagcagc agatgaaggt ctgctggcaa
      601 gcgatattgg tggtcagcag gatctggcac gtaccattgt tgcagcaacc attggtctgg
      661 aagcactgtg tcgtgataat ggtgaatggc tgagtccggg tacagttacc ggtctgtggc
      721 gtaccctgct gccgattgtt gccgcaccgg gtcgtagccc tccgtaagga tccaagcttg
      781 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      841 aggaagctga
//
