LOCUS       sequence-80180-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-HapR
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             179..202
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             845..862
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct
      181 ggaagttctg ttccaggggc cccatatgga tgcaagcatt gaaaaacgtc cgcgtacccg
      241 tctgagtccg cagaaacgta aactgcagct gatggaaatt gccctggaag tttttgcaaa
      301 acgtggtatt ggtcgtggtg gtcatgcaga tattgcagaa attgcacagg ttagcgttgc
      361 aaccgtgttt aactattttc cgacccgtga agatctggtt gatgatgttc tgaattttgt
      421 ggttcgccag tatagcaatt ttctgaccga tcacatcgat ctggatctgg atgttaaaac
      481 caatctgcag accgtttgca aagaaatggt taaactggca atgaccgatt gtcattggct
      541 gaaagtttgg tttgaatggt cagcaagcac ccgtgatgaa gtttggcctc tgtttgttag
      601 caccaatcgt accaatcagc tgctgattcg caacatgttt atgaaagcaa tggaacgtgg
      661 tgaactgtgc gaaaaacatg atgttgataa tatggccagc ctgtttcacg gtatctttta
      721 tagcatcttt ctgcaggtta atcgcctggg tgaacaagaa gcagtttata aactggcaga
      781 tagctatctg aacatgctgt gcatctataa aaactaagga tccaagcttg cggccgcact
      841 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
//
