LOCUS       sequence-80182-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-HemR
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             179..202
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct
      181 ggaagttctg ttccaggggc cccatatgga tgcaagcgat cataacgaat atttcagcgg
      241 tctggttttt ggtatgggtg gtgttgttgc agatgcagat ggtcagggca ccattccgcg
      301 tgaaacccgt cgtcgtggtc cggcactgga aaaagcaatt ctggatgcag gttggcgtga
      361 actggcaggc gcaggttatg ccggtctgac ctttgaagca gttgcagaac gtgcacatac
      421 cggtaaagca gcactgtatc gtcgttggcc gaataaagaa agcctggttc tggcagttct
      481 gagccagcgc tattttaacg atccggcaac cgttccggat accggtagcc tgcgtggtga
      541 tgttctggcc gttctgcgtg gtgcaaatcg tctgggtgat gatgcaccgg cactgattag
      601 cgttctgctg ggtgcatatt ttgatgaaac caataccaca ccgcaagaac tgcgtgcaca
      661 tctgctgggt gatcgtgttg ataccatggc actgctggtt gaacgtgcaa ttgcacgtgg
      721 tgaagttgca gcaccgctgc ctcgtcgtgt tctggcactg ccgatggatc tggttcgtct
      781 gcagtttttt atgacctttc agagcgttcc ggatgaagat attgttgata ttgtggatac
      841 cgtttttctg ccgct
//
