LOCUS       sequence-80183-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-HlyIIR
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    69..93
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             108..130
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             149..166
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             176..199
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
       61 actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt
      121 aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca gcggcctgga
      181 agttctgttc caggggcccc atatgaaata catcctgttt gaggtgtgcg aaatgggtaa
      241 aagccgtgaa cagaccatgg aaaatattct gaaagcagcc aaaaagaaat tcggcgaacg
      301 tggttatgaa ggcaccagca ttcaagaaat tgccaaagaa gccaaagtta acgttgcaat
      361 ggccagctat tactttaatg gcaaagagaa cctgtactac gaggtgttca aaaaatacgg
      421 tctggcaaat gaactgccga actttctgga aaaaaaccag tttaatccga ttaatgccct
      481 gcgtgaatat ctgaccgttt ttaccaccca cattaaagaa aatccggaaa ttggcaccct
      541 ggcctatgaa gaaattatca aagaaagcgc acgcctggaa aaaatcaaac cgtattttat
      601 cggcagcttc gaacagctga aagaaattct gcaagagggt gaaaaacagg gtgtgtttca
      661 cttttttagc atcaaccata ccatccattg gattaccagc attgttctgt ttccgaaatt
      721 caaaaaattc atcgatagcc tgggtccgaa tgaaaccaat gataccaatc atgaatggat
      781 gccggaagat ctggttagcc gtattattag cgcactgacc gataaaccga acatttaa
//
