LOCUS       sequence-80184-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for aDTX-lumitoxin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Schmidt D, Tillberg PW, Chen F, Boyden ES
  TITLE     A fully genetically encoded protein architecture for optical control
            of peptide ligand concentration.
  JOURNAL   Nat Commun. 2014 Jan 10;5:3019. doi: 10.1038/ncomms4019.
  PUBMED    24407101
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-01-10"; volume: "5:3019. doi"; pages: " 
            10.1038/ncomms4019"
COMMENT     Sequence Label: pcDNA3.1-aDTX-lumitoxin
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(142..288)
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             complement(310..738)
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
     CDS             complement(778..801)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc
       61 ctccatgtgc accttgaagc gcatgaactc cttgatgatg gccatgttat cctcctcgcc
      121 cttgctcacc atacgaatcc tacgtggttt cttctgccaa agcatgatga ggatgataag
      181 ggagatgatg gtgagcacca ccagggccag gatggctgag atcaccacca ccttaaaggg
      241 caaggagtgt ggcaccacga tgacctcctg cgtgtcctgg cccacagcaa ctcgagtacc
      301 gcggccgcta agttcttttg ccgcctcatc aatattttct gcagttttct taatcagcat
      361 gactccctct ctctcggcag catctcggac atgctcagtt ccatccaact gaaccccaat
      421 aaagtactgg acatctccct tctgatctcg cataggctgc aagtgaaaga ggttccagaa
      481 ctttttacca ctctttgtat aattaatcag ctgaacagtg acctctgttt ggttatctat
      541 ggcatctcta atttttctca ctgtcgcgcg atcagtttca ggaccttgta gaaacctgca
      601 gtttcttccc aaaatttctt cacggctata ttctgtcaac tgcaagaaac tatcggacgc
      661 gaatataatg ggattatctg gcaatcttgg gtcagtaatg acaaagttct tctcaatacg
      721 ttcaagtgta gtagccaaga attcgttgca cagggcgccg ccggcggcgg cgtcgatctt
      781 gtcgtcgtcg tccttgtagt cggcggcggc ggtaccgccg atgcaggttc ttctgcactc
      841 ctcgatggtc ttgaatctgt tgctgttgcc gccgcagccg ctccagtcga atctctcgca
      901 ctgcttcttc ttctggttgt agtagaaggc ggggatcttg tcgtagcatc tgccggggtt
      961 tctgtgcagg atgcacagct ttcttctggg ctgaagc
//
