LOCUS       sequence-80188-e        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for DTXK-lumitoxin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Schmidt D, Tillberg PW, Chen F, Boyden ES
  TITLE     A fully genetically encoded protein architecture for optical control
            of peptide ligand concentration.
  JOURNAL   Nat Commun. 2014 Jan 10;5:3019. doi: 10.1038/ncomms4019.
  PUBMED    24407101
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-01-10"; volume: "5:3019. doi"; pages: " 
            10.1038/ncomms4019"
COMMENT     Sequence Label: pcDNA3.1-DTXK-lumitoxin
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             144..167
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             363..386
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             426..854
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
     CDS             876..1022
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcat gagcgccctg ctgatcctgg
      121 ccctggtggg cgccgccgtg gccgactaca aggacgacga cgacaagctg aagcttgccg
      181 ccaagtactg caagctgccc ctgagaatcg gtccatgcaa gcggaagatc cccagcttct
      241 actacaagtg gaaggccaag cagtgcctgc ccttcgacta ctctggctgc ggaggcaacg
      301 ccaaccggtt caagaccatc gaggaatgca gacggacctg cgtgggaggt accgccgccg
      361 ccgactacaa ggacgacgac gacaagatcg acgccgccgc cggcggcgcc ctgtgcaacg
      421 aattcttggc tactacactt gaacgtattg agaagaactt tgtcattact gacccaagat
      481 tgccagataa tcccattata ttcgcgtccg atagtttctt gcagttgaca gaatatagcc
      541 gtgaagaaat tttgggaaga aactgcaggt ttctacaagg tcctgaaact gatcgcgcga
      601 cagtgagaaa aattagagat gccatagata accaaacaga ggtcactgtt cagctgatta
      661 attatacaaa gagtggtaaa aagttctgga acctctttca cttgcagcct atgcgagatc
      721 agaagggaga tgtccagtac tttattgggg ttcagttgga tggaactgag catgtccgag
      781 atgctgccga gagagaggga gtcatgctga ttaagaaaac tgcagaaaat attgatgagg
      841 cggcaaaaga acttagcggc cgcggtactc gagttgctgt gggccaggac acgcaggagg
      901 tcatcgtggt gccacactcc ttgcccttta aggtggtggt gatctcagcc atcctggccc
      961 tggtggtgct caccatcatc tcccttatca tcctcatcat gctttggcag aagaaaccac
     1021 gtaggattcg tatggtgagc aagggcgagg aggataacat ggccat
//
