LOCUS       sequence-80190-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for DTXK-lumitoxin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Schmidt D, Tillberg PW, Chen F, Boyden ES
  TITLE     A fully genetically encoded protein architecture for optical control
            of peptide ligand concentration.
  JOURNAL   Nat Commun. 2014 Jan 10;5:3019. doi: 10.1038/ncomms4019.
  PUBMED    24407101
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-01-10"; volume: "5:3019. doi"; pages: " 
            10.1038/ncomms4019"
COMMENT     Sequence Label: pcDNA3.1-DTXK-lumitoxin
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..731)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(299..318)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     568..586
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(741..887)
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
ORIGIN
        1 gtttaaacgg gccctctaga ttacttgtac agctcgtcca tgccgccggt ggagtggcgg
       61 ccctcggcgc gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc
      121 aacttgatgt tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg
      181 gtcttgacct cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg
      241 cccttcaggg cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc
      301 ttcttctgca ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag
      361 atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg
      421 aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg
      481 atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg
      541 tcccaggcga agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc
      601 tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc
      661 tccatgtgca ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc
      721 ttgctcacca tacgaatcct acgtggtttc ttctgccaaa gcatgatgag gatgataagg
      781 gagatgatgg tgagcaccac cagggccagg atggctgaga tcaccaccac cttaaagggc
      841 aaggagtgtg gcaccacgat gacctcctgc gtgtcctggc ccacagcaac tcgagtaccg
      901 cggccgctaa gttcttttgc cgcctcatca atattttctg cagttttctt aatcagc
//
