LOCUS       sequence-80191-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Heo I, Joo C, Cho J, Ha M, Han J, Kim VN
  TITLE     Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA.
  JOURNAL   Mol Cell. 2008 Oct 24;32(2):276-84. doi: 
            10.1016/j.molcel.2008.09.014.
  PUBMED    18951094
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2008.09"; journalName: "Mol Cell"; date: 
            "2008-10-24- 24"; volume: "32"; issue: "2"; pages: "276-84"
COMMENT     Sequence Label: pcDNA3-pri-let-7a-1
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(365..383)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(366..383)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(403..420)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    409..633
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(766..785)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt
      121 gctggaattc actagtgatt gattcctttt caccattcac cctggatgtt ctcttcactg
      181 tgggatgagg tagtaggttg tatagtttta gggtcacacc caccactggg agataactat
      241 acaatctact gtctttccta acgtgataga aaagtctgca tccaggcggt ctgatagaaa
      301 aatcgaattc tgcagatatc catcacactg gcggccgctc gagcatgcat ctagagggcc
      361 ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta
      421 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
      481 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
      541 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
      601 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
      661 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      721 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
      781 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
      841 cttta
//
