LOCUS       sequence-80193-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for generating dsDNA donors for homology-directed repair. 
            Contains the visible marker 3xP3-DsRed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Gratz SJ, Ukken FP, Rubinstein CD, Thiede G, Donohue LK, Cummings 
            AM, O'Connor-Giles KM
  TITLE     Highly specific and efficient CRISPR/Cas9-catalyzed 
            homology-directed repair in Drosophila.
  JOURNAL   Genetics. 2014 Apr;196(4):961-71. doi: 10.1534/genetics.113.160713. 
            Epub 2014 Jan 29.
  PUBMED    24478335
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.113.160713"; journalName: "Genetics"; date: 
            "2014-04"; volume: "196"; issue: "4"; pages: "961-71"
COMMENT     Sequence Label: pHD-DsRed
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    79..112
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      338..347
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(474..492)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(524..544)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     742..761
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttatggggtg tcgcccttcg ctgaagcagg tggaattctt gcatgctagc ggccgcggac
       61 atatgcacac ctgcgatcat aacttcgtat aatgtatgct atacgaagtt atcgtacggg
      121 atctaattca attagagact aattcaatta gagctaattc aattaggatc caagcttatc
      181 gatttcgaac cctcgaccgc cggagtataa atagaggcgc ttcgtctacg gagcgacaat
      241 tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg
      301 cagctgaaca agctaaacaa tcggctcgaa gccggtcgcc accatggcct cctccgagga
      361 cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga
      421 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      481 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca
      541 gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc
      601 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
      661 cgtgacccag gactcctccc tccaggacgg ctccttcatc tacaaggtga agttcatcgg
      721 cgtgaacttc ccctccgacg gccccgtaat gcagaagaag actatgggct gggaggcgtc
      781 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccaca aggccctgaa
      841 gctgaaggac ggcggccact acctggtgga gttcaagtcc atctacatgg ccaagaagcc
      901 cgtgcagctg cccggctact actacgtgga ctccaagctg gacatcacct ccc
//
