LOCUS       sequence-80195-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for generating dsDNA donors for homology-directed repair. 
            Contains the visible marker 3xP3-DsRed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Gratz SJ, Ukken FP, Rubinstein CD, Thiede G, Donohue LK, Cummings 
            AM, O'Connor-Giles KM
  TITLE     Highly specific and efficient CRISPR/Cas9-catalyzed 
            homology-directed repair in Drosophila.
  JOURNAL   Genetics. 2014 Apr;196(4):961-71. doi: 10.1534/genetics.113.160713. 
            Epub 2014 Jan 29.
  PUBMED    24478335
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.113.160713"; journalName: "Genetics"; date: 
            "2014-04"; volume: "196"; issue: "4"; pages: "961-71"
COMMENT     Sequence Label: pHD-DsRed
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    162..283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(199..218)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     253..272
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    290..323
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(859..876)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(859..875)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtacgagcg cgccgagggc cgccaccacc tgttcctgta ggggccgcga ctctagatca
       61 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
      121 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
      181 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      241 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaccggta taacttcgta
      301 taatgtatgc tatacgaagt tatagaagag cactagtaaa gatctccatg cataaggcgc
      361 gcctaggcct tctgcagctc gaggctcttc cgtcaatcga gttcaagggc gacacaaaat
      421 ttattctaaa tgcataataa atactgataa catcttatag tttgtattat attttgtatt
      481 atcgttgaca tgtataattt tgatatcaaa aactgatttt ccctttatta ttttcgagat
      541 ttattttctt aattctcttt aacaaactag aaatattgta tatacaaaaa atcataaata
      601 atagatgaat agtttaatta taggtgttca tcaatcgaaa aagcaacgta tcttatttaa
      661 agtgcgttgc ttttttctca tttataaggt taaataattc tcatatatca agcaaagtga
      721 caggcgccct taaatattct gacaaatgct ctttccctaa actcccccca taaaaaaacc
      781 cgccgaagcg ggtttttacg ttatttgcgg attaacgatt actcgttatc agaaccgccc
      841 agggggcccg agcttaagac tggccgtcgt tttacaacac agaaagagtt tgtagaaacg
      901 caaaaaggcc atccgtcagg ggccttctgc
//
