LOCUS       sequence-80199-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Forebrain principle neuron specific expression of GCaMP6f Ca sensor 
            and physically separate nuclear localized dTomato fluorophore .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  TITLE     Ting GCaMP6 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..772)
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(157..180)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(355..374)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     624..642
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(773..793)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(806..862)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattccc tgcaggggcg
       61 cgccgtcgac ttacttgtac agctcgtcca tgccgtacag gaacaggtgg tggcggccct
      121 cggagcgctc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg atgtccagct
      181 tggtgtccac gtagtagtag ccgggcagtt gcacgggctt cttggccatg tagatggtct
      241 tgaactccac caggtagtgg ccgccgtcct tcagcttcag ggcctggtgg atctcgccct
      301 tcagcacgcc gtcgcggggg tacaggcgct cggtggaggc ctcccagccc atggtcttct
      361 tctgcattac ggggccgtcg ggggggaagt tggtgccgcg catcttcacc ttgtagatca
      421 gcgtgccgtc ctgcagggag gagtcctggg tcacggtcac cagaccgccg tcctcgaagt
      481 tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcttgtaa tcggggatgt
      541 cggcggggtg cttcacgtac gccttggagc cgtacatgaa ctggggggac aggatgtccc
      601 aggcgaaggg cagggggccg cccttggtca ccttcagctt ggcggtctgg gtgccctcgt
      661 aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcatg gagccctcca
      721 tgcgcacctt gaagcgcatg aactctttga tgacctcctc gcccttgctc accaccttcc
      781 tcttcttctt gggggccatt ctagatggcc cgggattctc ttcgacatcc cctgcttgtt
      841 tcaacaggga gaagttagtg gctccgcttc cgttatcctt cgctgtcatc atttgtacaa
      901 actcttcgta
//
