LOCUS       sequence-80201-e        1116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  vertebrate expression of codon-optimized Gal80 with N-terminal NLS 
            and no stop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Fujimoto E, Gaynes B, Brimley CJ, Chien CB, Bonkowsky JL
  TITLE     Gal80 intersectional regulation of cell-type specific expression in 
            vertebrates.
  JOURNAL   Dev Dyn. 2011 Oct;240(10):2324-34. doi: 10.1002/dvdy.22734. Epub 
            2011 Sep 8.
  PUBMED    21905164
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/dvdy.22734"; journalName: "Dev Dyn"; date: "2011-10"; 
            volume: "240"; issue: "10"; pages: "2324-34"
COMMENT     Sequence Label: pME-nlsGal80(opt)
FEATURES             Location/Qualifiers
     source          1..1116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..117
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             129..149
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtcttaagct cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
       61 aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggctgta
      121 ccatggctcc aaagaagaag cgtaaggtaa tggactacaa caaaaggagc agtgtctcaa
      181 cagtgccaaa cgccgcccct attagagtgg gattcgtggg tctgaacgca gcaaagggat
      241 gggccattaa gacccactac cctgcaatcc tgcagctgag ctctcagttc cagattactg
      301 ctctgtattc tccaaagatc gaaacatcca ttgccaccat ccagagactg aaactgtcta
      361 acgctactgc cttcccaaca ctggagtcct ttgcttcctc aagtaccatc gacatgatcg
      421 tgattgctat ccaggtcgcc tcacattacg aggtggtcat gcccctgctg gagttcagca
      481 agaacaatcc taacctgaaa tatctgtttg tggagtgggc actggcttgt tcactggatc
      541 aggccgagag tatctacaaa gctgccgcag aacgcggcgt ccagactatc attagcctgc
      601 agggtcgcaa gtctccctac attctgagag ccaaagagct gatcagtcag ggatatattg
      661 gcgacatcaa cagcattgag atcgctggta atggaggctg gtacggatat gaaagaccag
      721 tgaagagccc caaatacatt tatgagatcg gcaacggtgt ggacctggtc acaaccactt
      781 tcggacacac aattgacatc ctgcagtaca tgaccagctc ttatttcagc agaatcaacg
      841 ctatggtgtt taacaatatt cctgagcagg aactgatcga cgagagagga aataggctgg
      901 gccagagggt gcctaagact gtcccagatc acctgctgtt tcagggcaca ctgctgaacg
      961 gtaatgtgcc agtctcctgc tcattcaagg gtggaaaacc caccaagaaa tttactaaaa
     1021 acctggtgat tgacatccac ggaacaaagg gagacctgaa actggagggc gatgccggtt
     1081 tcgcagaaat ctccaatctg gtgctgtact attcag
//
