LOCUS       sequence-80205-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-CasR
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    79..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             118..140
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             159..176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             186..209
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
       61 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
      121 gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca
      181 gcggcctgga agttctgttc caggggcccc atatgaaagc aattgatggt ccggcagatg
      241 aaccgcgtcg tcgtggtcgt ccgaaagtta gcaccgatga agataaacgt gtgcagattg
      301 ttgaagttgc acgtcgtgtg tttgtgaaat atggttatgc aggtagcacc accgcagttg
      361 ttgcaagcga agccggtgtt agcaaacaga ccctgtatcg tctgtttgaa agcaaagaag
      421 aactgtttgc agcagttgtt gccgcacatc gtcgtctgat gctggatctg cctcgtccgg
      481 cagaagattg tagcattgca gaaagcctgg aacgcatttt tatgctggat atggatgaag
      541 atcgtgatgc agatcgtgca ggttttctgc agctggtttt tcgtgaagca gcacagtttc
      601 cggaactggt tgatattctg cagcgtgaag gtatgctggc aagccgtcaa gaactggcag
      661 attggctgag cgatcgtcgt gcagaacgtg tttatccggg tcgtagcggt aaacgtcgtc
      721 cggatgcaga tggtcatgat ctgcgtcgtc atggtacagc cggtggtccg tgtagcggtc
      781 tggcacaggc aggtagcgca
//
